Detailed information of TA system TA028630

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 312663..313218
• Replicon: plasmid pRmeGR4d
• Accession: NC_019849
• Organism: Sinorhizobium meliloti GR4

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: C770_RS43495
• Protein ID: WP_041169867.1
• Coordinates: 312901..313218 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: H0FXK0
• Locus tag: C770_RS43490
• Protein ID: WP_003527850.1
• Coordinates: 312663..312911 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C770_RS43470	308893..310224	+	1332	WP_015242827.1	O-antigen ligase family protein	-
C770_RS43475	310217..310972	+	756	WP_015242828.1	polysaccharide deacetylase family protein	-
C770_RS43480	310983..312212	+	1230	WP_015242829.1	glycosyltransferase family 4 protein	-
C770_RS43485	312329..312556	+	228	WP_015242830.1	hypothetical protein	-
C770_RS43490	312663..312911	+	249	WP_003527850.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
C770_RS43495	312901..313218	+	318	WP_041169867.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C770_RS52145	313189..313389	-	201	Protein_280	helix-turn-helix domain-containing protein	-
C770_RS43500	313711..314880	+	1170	WP_015242832.1	glycosyltransferase family 4 protein	-
C770_RS43505	314877..315266	+	390	WP_015242833.1	hypothetical protein	-
C770_RS43510	315297..316340	+	1044	WP_015242834.1	glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..1701381	1701381

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-93)

Antitoxin

• Antitoxin ParD (3-78)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12255.06 Da
• Isoelectric Point: 11.0222

>T28630 WP_041169867.1 NC_019849:312901-313218 [Sinorhizobium meliloti GR4]
MATERPFRLAPAAKADLRKIWRHTARRWSLEQAETYQDQLYTAFEGLAAGTKKGRNVDVRPGYLKYPAGAHIVYFRDRGD
RIDIIRILHGRMDAQRTHRAYMRAP

Nucleotide

• Length: 318 bp

>T28630 NC_019849:312901-313218 [Sinorhizobium meliloti GR4]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTGCCGCCAAGGCCGACCTTCGGAAGATATGGCGCCACACGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGGCTGGCCGCAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGGCCGGGTTATCTCAAATACCCGGCAGGAGCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGGATGGACGCCCAACGAACGCATCGGGCTTATATGCGGGCGCCGTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8888.73 Da
• Isoelectric Point: 4.2176

>AT28630 WP_003527850.1 NC_019849:312663-312911 [Sinorhizobium meliloti GR4]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Nucleotide

• Length: 249 bp

>AT28630 NC_019849:312663-312911 [Sinorhizobium meliloti GR4]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CTTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Structures

Antitoxin

• AlphaFold DB: H0FXK0