Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2700973..2702561 Replicon chromosome
Accession NZ_LR130539
Organism Klebsiella variicola strain AJ055 isolate AJ055

Toxin (Protein)


Gene name hipA Uniprot ID A0A1F2MCM0
Locus tag EW050_RS13325 Protein ID WP_008804919.1
Coordinates 2700973..2702295 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A1F2MCN9
Locus tag EW050_RS13330 Protein ID WP_008804920.1
Coordinates 2702295..2702561 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW050_RS13310 2696038..2698539 + 2502 WP_022066169.1 fimbrial biogenesis outer membrane usher protein -
EW050_RS13315 2698527..2699531 + 1005 WP_032730346.1 type 1 fimbrial protein -
EW050_RS13320 2699664..2700956 + 1293 WP_012968243.1 glycoside hydrolase family 10 protein -
EW050_RS13325 2700973..2702295 - 1323 WP_008804919.1 type II toxin-antitoxin system HipA family toxin Toxin
EW050_RS13330 2702295..2702561 - 267 WP_008804920.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
EW050_RS13335 2702827..2703153 - 327 WP_008804921.1 hydroxyisourate hydrolase -
EW050_RS13340 2703150..2703650 - 501 WP_008804922.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
EW050_RS13345 2703628..2705007 - 1380 WP_110184980.1 purine permease -
EW050_RS13350 2705353..2706507 + 1155 WP_110184979.1 FAD-dependent urate hydroxylase HpxO -
EW050_RS13355 2706488..2707411 - 924 WP_032754373.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(13-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 48911.26 Da        Isoelectric Point: 7.9109

>T286294 WP_008804919.1 NZ_LR130539:c2702295-2700973 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGEQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9936.30 Da        Isoelectric Point: 8.5912

>AT286294 WP_008804920.1 NZ_LR130539:c2702561-2702295 [Klebsiella variicola]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2MCM0

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MCN9

References