Detailed information of TA system TA028629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1360855..1361525
• Replicon: plasmid pRmeGR4c
• Accession: NC_019848
• Organism: Sinorhizobium meliloti GR4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C770_RS41820
• Protein ID: WP_015242680.1
• Coordinates: 1360855..1361274 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6A7ZM99
• Locus tag: C770_RS41825
• Protein ID: WP_015242681.1
• Coordinates: 1361271..1361525 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C770_RS41805	1355916..1357076	+	1161	WP_015242676.1	GGDEF domain-containing protein	-
C770_RS41810	1357133..1357495	-	363	WP_015242677.1	response regulator	-
C770_RS41815	1357523..1360057	-	2535	WP_041169808.1	PAS domain S-box protein	-
C770_RS52050	1360458..1360886	+	429	WP_153315244.1	GFA family protein	-
C770_RS41820	1360855..1361274	-	420	WP_015242680.1	type II toxin-antitoxin system VapC family toxin	Toxin
C770_RS41825	1361271..1361525	-	255	WP_015242681.1	plasmid stability protein	Antitoxin
C770_RS41830	1361718..1362680	-	963	WP_015242682.1	ABC transporter ATP-binding protein	-
C770_RS41835	1362677..1363264	-	588	Protein_1267	ABC transporter ATP-binding protein	-
C770_RS53875	1363260..1363724	+	465	WP_015242684.1	hypothetical protein	-
C770_RS41845	1363761..1365353	-	1593	WP_015242685.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1417907	1417907

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14761.01 Da
• Isoelectric Point: 6.7266

>T28629 WP_015242680.1 NC_019848:c1361274-1360855 [Sinorhizobium meliloti GR4]
MIVLGTNVVSEAMKPTPDPAVRSWLNEQVAETLYLASVTLAELLFGIGALPDGRRKKALTEMLGGVLELFGDRVLPFDIG
AARHYAELAVTARAAGKGFPTADGYIAAIAASRGFIVATRDSSPFEAAGLTVVNPWHHR

Nucleotide

• Length: 420 bp

>T28629 NC_019848:c1361274-1360855 [Sinorhizobium meliloti GR4]
ATGATTGTCCTCGGTACGAATGTCGTCTCCGAGGCGATGAAACCGACCCCCGATCCGGCCGTGCGCTCCTGGTTGAACGA
GCAGGTCGCTGAAACCTTGTATCTGGCCAGTGTGACCTTGGCCGAACTGCTGTTCGGCATCGGTGCGCTGCCGGACGGCC
GGCGCAAGAAGGCTCTGACCGAAATGCTTGGCGGCGTGCTCGAACTGTTTGGCGATCGCGTATTGCCGTTCGACATCGGA
GCTGCTCGCCATTATGCCGAGCTTGCGGTGACCGCTCGAGCGGCCGGCAAGGGATTTCCGACGGCCGACGGATATATTGC
CGCCATTGCCGCCTCCAGGGGATTTATCGTCGCGACCCGCGATAGCAGCCCGTTCGAAGCGGCTGGACTTACCGTGGTAA
ATCCTTGGCACCATCGATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9480.82 Da
• Isoelectric Point: 6.4732

>AT28629 WP_015242681.1 NC_019848:c1361525-1361271 [Sinorhizobium meliloti GR4]
MAILTVRNVPDEVHRALRVRAAMHGRSTEAEVREILESAVKPEQRVRMGDALAELGRRLGLTNDDFAVFDEVRDKAPAQP
VRFE

Nucleotide

• Length: 255 bp

>AT28629 NC_019848:c1361525-1361271 [Sinorhizobium meliloti GR4]
ATGGCGATCCTGACGGTCCGGAATGTGCCCGATGAAGTGCATCGCGCCTTGCGCGTGAGAGCGGCGATGCACGGCCGCAG
CACCGAGGCGGAAGTCCGGGAGATTTTGGAAAGTGCGGTGAAGCCCGAGCAGCGCGTTCGCATGGGCGATGCCCTGGCCG
AACTCGGCCGGCGACTGGGGCTGACCAATGACGATTTCGCCGTGTTCGACGAGGTACGCGACAAGGCGCCGGCGCAGCCC
GTGAGATTTGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6A7ZM99