Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1792085..1792675 Replicon chromosome
Accession NZ_LR130539
Organism Klebsiella variicola strain AJ055 isolate AJ055

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag EW050_RS08775 Protein ID WP_008804165.1
Coordinates 1792343..1792675 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag EW050_RS08770 Protein ID WP_012541132.1
Coordinates 1792085..1792342 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW050_RS08745 1787692..1788267 + 576 WP_012541129.1 hypothetical protein -
EW050_RS08750 1788446..1789282 + 837 WP_008804155.1 alpha/beta hydrolase -
EW050_RS08755 1789489..1790460 + 972 WP_023340477.1 sensor domain-containing diguanylate cyclase -
EW050_RS08760 1790457..1791557 - 1101 WP_023340476.1 hypothetical protein -
EW050_RS08770 1792085..1792342 + 258 WP_012541132.1 antitoxin Antitoxin
EW050_RS08775 1792343..1792675 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EW050_RS08785 1792998..1794434 + 1437 WP_016161429.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
EW050_RS08795 1794807..1796261 - 1455 WP_087498504.1 AMP nucleosidase -
EW050_RS08800 1796392..1796637 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T286289 WP_008804165.1 NZ_LR130539:1792343-1792675 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT286289 WP_012541132.1 NZ_LR130539:1792085-1792342 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References