Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 739143..739918 Replicon chromosome
Accession NZ_LR130539
Organism Klebsiella variicola strain AJ055 isolate AJ055

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A2N4Z6P0
Locus tag EW050_RS03670 Protein ID WP_032740995.1
Coordinates 739433..739918 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag EW050_RS03665 Protein ID WP_004150912.1
Coordinates 739143..739436 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW050_RS03645 734399..735001 - 603 WP_012540553.1 short chain dehydrogenase -
EW050_RS03650 735099..736010 + 912 WP_012540554.1 LysR family transcriptional regulator -
EW050_RS03655 736011..737159 - 1149 WP_008806488.1 PLP-dependent aspartate aminotransferase family protein -
EW050_RS03660 737171..738547 - 1377 WP_016161835.1 cystathionine beta-synthase -
EW050_RS03665 739143..739436 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
EW050_RS03670 739433..739918 + 486 WP_032740995.1 GNAT family N-acetyltransferase Toxin
EW050_RS03675 740622..741215 + 594 WP_110185300.1 hypothetical protein -
EW050_RS03680 741312..741528 + 217 Protein_714 transposase -
EW050_RS03695 742051..742764 - 714 WP_008806478.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 741312..741464 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17618.64 Da        Isoelectric Point: 8.5178

>T286287 WP_032740995.1 NZ_LR130539:739433-739918 [Klebsiella variicola]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVH
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT286287 WP_004150912.1 NZ_LR130539:739143-739436 [Klebsiella variicola]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N4Z6P0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References