Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5367113..5367738 Replicon chromosome
Accession NZ_LR130538
Organism Klebsiella variicola strain AJ292 isolate AJ292

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag EW044_RS26300 Protein ID WP_008807903.1
Coordinates 5367113..5367496 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag EW044_RS26305 Protein ID WP_004150355.1
Coordinates 5367496..5367738 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW044_RS26285 5364479..5365381 + 903 WP_129676323.1 formate dehydrogenase subunit beta -
EW044_RS26290 5365378..5366013 + 636 WP_129676326.1 formate dehydrogenase cytochrome b556 subunit -
EW044_RS26295 5366010..5366939 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
EW044_RS26300 5367113..5367496 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
EW044_RS26305 5367496..5367738 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
EW044_RS26310 5367943..5368860 + 918 WP_023296845.1 alpha/beta hydrolase -
EW044_RS26315 5368875..5369816 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
EW044_RS26320 5369861..5370298 - 438 WP_023296844.1 D-tyrosyl-tRNA(Tyr) deacylase -
EW044_RS26325 5370295..5371155 - 861 WP_008807907.1 virulence factor BrkB family protein -
EW044_RS26330 5371149..5371748 - 600 WP_129676329.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T286284 WP_008807903.1 NZ_LR130538:c5367496-5367113 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT286284 WP_004150355.1 NZ_LR130538:c5367738-5367496 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References