Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1230743..1231323 Replicon plasmid pRmeGR4c
Accession NC_019848
Organism Sinorhizobium meliloti GR4

Toxin (Protein)


Gene name vapC Uniprot ID Q92XP1
Locus tag C770_RS41185 Protein ID WP_010968151.1
Coordinates 1230940..1231323 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID F7XCL0
Locus tag C770_RS41180 Protein ID WP_013851145.1
Coordinates 1230743..1230943 (+) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C770_RS41170 1228254..1229249 + 996 WP_015242577.1 SLAC1 anion channel family protein -
C770_RS41175 1229249..1230208 + 960 WP_015242578.1 GlxA family transcriptional regulator -
C770_RS52010 1230303..1230548 + 246 WP_014532040.1 membrane protein -
C770_RS41180 1230743..1230943 + 201 WP_013851145.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
C770_RS41185 1230940..1231323 + 384 WP_010968151.1 type II toxin-antitoxin system VapC family toxin Toxin
C770_RS41190 1231771..1232166 + 396 WP_041169861.1 response regulator -
C770_RS41195 1232450..1232761 - 312 WP_015242581.1 hypothetical protein -
C770_RS41200 1233094..1233930 + 837 WP_015242582.1 TIGR02587 family membrane protein -
C770_RS41205 1233927..1234373 + 447 WP_015242583.1 TIGR02588 family protein -
C770_RS41210 1234542..1234946 - 405 WP_015242584.1 MerR family DNA-binding protein -
C770_RS41215 1235001..1235789 + 789 WP_015242585.1 glutaredoxin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB 1..1417907 1417907


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14292.54 Da        Isoelectric Point: 6.9172

>T28628 WP_010968151.1 NC_019848:1230940-1231323 [Sinorhizobium meliloti GR4]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Download         Length: 384 bp

>T28628 NC_019848:1230940-1231323 [Sinorhizobium meliloti GR4]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATGATGATCGATCGCCACGCCATTTTTAGCATG
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACGCGGAACTGA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7096.22 Da        Isoelectric Point: 11.1107

>AT28628 WP_013851145.1 NC_019848:1230743-1230943 [Sinorhizobium meliloti GR4]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT28628 NC_019848:1230743-1230943 [Sinorhizobium meliloti GR4]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGTCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92XP1

Antitoxin

Source ID Structure
AlphaFold DB A0A7W6MID2

References