Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 273740..274371 Replicon chromosome
Accession NZ_LR130538
Organism Klebsiella variicola strain AJ292 isolate AJ292

Toxin (Protein)


Gene name hicA Uniprot ID A0A7H4S6Z2
Locus tag EW044_RS01250 Protein ID WP_008806943.1
Coordinates 273740..274015 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7H4S6Z1
Locus tag EW044_RS01255 Protein ID WP_023298308.1
Coordinates 274012..274371 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EW044_RS01230 268976..269311 + 336 WP_002921203.1 universal stress protein UspB -
EW044_RS01235 269371..270867 - 1497 WP_008806938.1 inorganic phosphate transporter PitA -
EW044_RS01240 271097..272290 + 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
EW044_RS01245 272330..273343 - 1014 WP_012540371.1 magnesium transporter -
EW044_RS01250 273740..274015 + 276 WP_008806943.1 type II toxin-antitoxin system HicA family toxin Toxin
EW044_RS01255 274012..274371 + 360 WP_023298308.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EW044_RS01260 274399..274800 - 402 WP_023298307.1 nickel-responsive transcriptional regulator NikR -
EW044_RS01265 274788..275579 - 792 WP_012540373.1 nickel import ATP-binding protein NikE -
EW044_RS01270 275576..276340 - 765 WP_008806946.1 nickel import ATP-binding protein NikD -
EW044_RS01275 276340..277173 - 834 WP_012967107.1 nickel ABC transporter permease subunit NikC -
EW044_RS01280 277170..278114 - 945 WP_016161924.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10295.87 Da        Isoelectric Point: 10.3900

>T286272 WP_008806943.1 NZ_LR130538:273740-274015 [Klebsiella variicola]
MEQQVLSLRNKQRHTLEQLFKTPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVRP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13341.08 Da        Isoelectric Point: 4.4605

>AT286272 WP_023298308.1 NZ_LR130538:274012-274371 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNVFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H4S6Z2

Antitoxin

Source ID Structure
AlphaFold DB A0A7H4S6Z1

References