Detailed information of TA system TA286244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 5006460..5008155
• Replicon: chromosome
• Accession: NZ_LR130533
• Organism: Pseudomonas aeruginosa isolate paerg009

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: EJN60_RS24055
• Protein ID: WP_023095293.1
• Coordinates: 5006460..5007329 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: EJN60_RS24060
• Protein ID: WP_003112902.1
• Coordinates: 5007322..5008155 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJN60_RS24030	5002468..5003319	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
EJN60_RS24035	5003360..5004367	+	1008	WP_022579704.1	ABC transporter substrate-binding protein	-
EJN60_RS24040	5004364..5005140	+	777	WP_003112898.1	ABC transporter permease	-
EJN60_RS24045	5005146..5005907	+	762	WP_003112899.1	SDR family oxidoreductase	-
EJN60_RS24050	5005921..5006451	+	531	WP_003112900.1	cupin domain-containing protein	-
EJN60_RS24055	5006460..5007329	+	870	WP_023095293.1	alpha/beta hydrolase	Toxin
EJN60_RS24060	5007322..5008155	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
EJN60_RS24065	5008142..5008939	+	798	WP_022579702.1	SDR family oxidoreductase	-
EJN60_RS24070	5008932..5010613	+	1682	Protein_4621	thiamine pyrophosphate-binding protein	-
EJN60_RS24075	5010624..5011427	+	804	WP_003112905.1	aspartate dehydrogenase	-
EJN60_RS24080	5011439..5012923	+	1485	WP_022579701.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30379.61 Da
• Isoelectric Point: 6.8139

>T286244 WP_023095293.1 NZ_LR130533:5006460-5007329 [Pseudomonas aeruginosa]
MNMAQTHIPAPSLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAARLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERLGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSPISGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT286244 WP_003112902.1 NZ_LR130533:5007322-5008155 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

Structures

Antitoxin

• AlphaFold DB: Q9HYA1