Detailed information of TA system TA028624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2924782..2925413
• Replicon: chromosome
• Accession: NC_019845
• Organism: Sinorhizobium meliloti GR4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C770_RS13825
• Protein ID: WP_013844828.1
• Coordinates: 2924782..2925180 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92MD9
• Locus tag: C770_RS13830
• Protein ID: WP_010970129.1
• Coordinates: 2925180..2925413 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C770_RS13795	2919864..2920166	-	303	WP_010970122.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C770_RS13800	2920163..2920438	-	276	WP_010970123.1	type II toxin-antitoxin system ParD family antitoxin	-
C770_RS50565	2920724..2920873	+	150	WP_010970124.1	hypothetical protein	-
C770_RS13805	2920870..2921805	+	936	WP_010970125.1	site-specific tyrosine recombinase XerD	-
C770_RS13810	2921869..2922822	+	954	WP_003527479.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha	-
C770_RS13815	2923067..2924509	+	1443	WP_010970126.1	murein L,D-transpeptidase	-
C770_RS13820	2924509..2924748	+	240	WP_010970127.1	sulfurtransferase TusA family protein	-
C770_RS13825	2924782..2925180	-	399	WP_013844828.1	type II toxin-antitoxin system toxin VapC	Toxin
C770_RS13830	2925180..2925413	-	234	WP_010970129.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C770_RS13835	2925522..2926667	-	1146	WP_010970130.1	GTP-binding protein	-
C770_RS13840	2926681..2927763	-	1083	WP_010970131.1	D-alanyl-D-alanine carboxypeptidase	-
C770_RS13845	2928015..2929178	+	1164	WP_010970132.1	amidohydrolase	-
C770_RS13850	2929201..2929665	+	465	WP_010970133.1	Lrp/AsnC ligand binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14829.13 Da
• Isoelectric Point: 6.9581

>T28624 WP_013844828.1 NC_019845:c2925180-2924782 [Sinorhizobium meliloti GR4]
MLTYMLDTNICIYVMKTYPPAVREKFNGLAEQLCISSITLGELHYGAEKSARRVENLTAIEHFVARLEVLPFADKAAAHY
GQVRAELERTGTPCGPHDMQIGAHARSEGLIVVTNNIREFVRMPGVRVENWL

Nucleotide

• Length: 399 bp

>T28624 NC_019845:c2925180-2924782 [Sinorhizobium meliloti GR4]
ATGCTCACCTATATGCTCGATACCAATATCTGCATCTATGTGATGAAGACCTATCCGCCGGCCGTGCGCGAAAAGTTCAA
CGGACTGGCGGAGCAGCTTTGCATATCGAGCATCACGCTGGGAGAATTACACTATGGCGCCGAAAAATCGGCCCGGCGCG
TTGAGAACCTTACGGCTATCGAACATTTCGTGGCACGGCTGGAGGTGCTGCCGTTCGCAGACAAGGCAGCGGCCCACTAC
GGGCAGGTTCGGGCTGAACTGGAGCGGACGGGAACGCCATGCGGCCCTCATGACATGCAGATCGGCGCGCATGCGCGCAG
CGAAGGGCTGATTGTCGTGACCAACAATATACGCGAGTTCGTCCGCATGCCGGGTGTAAGGGTCGAGAACTGGCTGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8673.87 Da
• Isoelectric Point: 8.9167

>AT28624 WP_010970129.1 NC_019845:c2925413-2925180 [Sinorhizobium meliloti GR4]
MASSTVFISNRSQAVRLPKAVAFPEGVHQVDILKIGRSRVIVPQGKRWDDLFLSGPRVSEDFMSERDQPVAETRESF

Nucleotide

• Length: 234 bp

>AT28624 NC_019845:c2925413-2925180 [Sinorhizobium meliloti GR4]
TTGGCCAGTTCGACCGTATTCATCAGTAACCGTAGCCAGGCGGTGCGGCTGCCAAAGGCCGTCGCCTTTCCCGAGGGCGT
GCATCAGGTCGATATCCTCAAGATCGGCCGCAGCCGCGTGATCGTCCCCCAGGGCAAAAGATGGGACGATCTGTTCCTCA
GCGGGCCGCGCGTCAGCGAGGATTTCATGAGCGAGCGTGATCAGCCGGTGGCGGAAACACGCGAATCCTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q92MD9