Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4509555..4510355 Replicon chromosome
Accession NZ_LR130532
Organism Escherichia coli isolate ecoli019

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag EJK95_RS22155 Protein ID WP_000342449.1
Coordinates 4509555..4510082 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag EJK95_RS22160 Protein ID WP_001277108.1
Coordinates 4510089..4510355 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJK95_RS22130 4504632..4505399 - 768 WP_000082110.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
EJK95_RS22135 4505396..4506673 - 1278 WP_000803782.1 branched chain amino acid ABC transporter permease LivM -
EJK95_RS22140 4506670..4507596 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
EJK95_RS22145 4507644..4508753 - 1110 WP_096957216.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
EJK95_RS22150 4509175..4509558 + 384 WP_000778785.1 aspartate 1-decarboxylase autocleavage activator PanM -
EJK95_RS22155 4509555..4510082 - 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
EJK95_RS22160 4510089..4510355 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
EJK95_RS22165 4510505..4511608 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
EJK95_RS22170 4511879..4512733 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
EJK95_RS22175 4512978..4514036 - 1059 WP_096845886.1 permease-like cell division protein FtsX -
EJK95_RS22180 4514029..4514697 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T286237 WP_000342449.1 NZ_LR130532:c4510082-4509555 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT286237 WP_001277108.1 NZ_LR130532:c4510355-4510089 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References