Detailed information of TA system TA028623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 2877235..2878001
• Replicon: chromosome
• Accession: NC_019845
• Organism: Sinorhizobium meliloti GR4

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: H0G6D3
• Locus tag: C770_RS13565
• Protein ID: WP_003533488.1
• Coordinates: 2877519..2878001 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: H0G6D4
• Locus tag: C770_RS13560
• Protein ID: WP_003533490.1
• Coordinates: 2877235..2877519 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C770_RS13545	2872705..2874093	+	1389	WP_003533497.1	lipopolysaccharide biosynthesis protein	-
C770_RS13550	2874097..2875368	+	1272	WP_010970087.1	GNAT family N-acetyltransferase	-
C770_RS13555	2875384..2877030	-	1647	WP_010970088.1	AMP-binding protein	-
C770_RS13560	2877235..2877519	+	285	WP_003533490.1	DUF1778 domain-containing protein	Antitoxin
C770_RS13565	2877519..2878001	+	483	WP_003533488.1	GNAT family N-acetyltransferase	Toxin
C770_RS13575	2878466..2878975	+	510	WP_013844819.1	disulfide bond formation protein B	-
C770_RS13580	2879007..2879564	-	558	WP_003533486.1	HNH endonuclease	-
C770_RS13585	2879670..2880317	-	648	WP_015241323.1	DNA-3-methyladenine glycosylase	-
C770_RS13590	2880470..2881366	+	897	WP_010970092.1	tRNA glutamyl-Q(34) synthetase GluQRS	-
C770_RS13595	2881385..2882260	-	876	WP_010970093.1	YihY/virulence factor BrkB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17463.88 Da
• Isoelectric Point: 7.3827

>T28623 WP_003533488.1 NC_019845:2877519-2878001 [Sinorhizobium meliloti GR4]
MLSAPTPLGEAHDFDLFQSGNDTLDDWLRRRAHANQASGASRTYVIAEEWRVVGYYCLASGALDLADAPSSVRRNMPDPI
PMAVLGRLAIDRDWQGKGLGAALLQDAVLRSSQAADIMGIRGLLVHAISGEAKAFYEHYGFQCSPNHPMTLVLSLKGKRR

Nucleotide

• Length: 483 bp

>T28623 NC_019845:2877519-2878001 [Sinorhizobium meliloti GR4]
ATGCTGTCGGCGCCGACGCCTCTTGGTGAAGCCCACGACTTCGATCTCTTCCAGAGCGGCAACGACACGCTCGATGACTG
GCTGCGCCGCCGTGCGCACGCTAATCAGGCCAGTGGCGCGTCGCGCACCTATGTTATCGCTGAAGAATGGCGCGTGGTCG
GCTATTACTGCCTTGCATCCGGTGCCCTCGATCTGGCCGACGCGCCGAGCTCTGTCCGGCGCAACATGCCGGATCCCATC
CCGATGGCGGTGCTCGGTCGCCTTGCTATCGACCGCGACTGGCAGGGCAAAGGGTTGGGCGCGGCGCTATTGCAAGATGC
CGTCCTGCGGTCCTCGCAGGCGGCCGACATCATGGGAATACGGGGCTTGCTCGTGCATGCCATCTCGGGCGAGGCGAAAG
CCTTTTACGAACATTACGGCTTCCAGTGCTCACCCAACCATCCCATGACGCTGGTGTTGTCGCTCAAGGGCAAGCGCCGT
TAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10771.12 Da
• Isoelectric Point: 7.3175

>AT28623 WP_003533490.1 NC_019845:2877235-2877519 [Sinorhizobium meliloti GR4]
MATKSSTDTHAKPVNLRIREDIRKVIDRAASIRGKTRSDFMIDAAYRAAEDTLLDQTLVRVDEHSYRHYLDILDQPPSGD
GFERLMNAPKPWQS

Nucleotide

• Length: 285 bp

>AT28623 NC_019845:2877235-2877519 [Sinorhizobium meliloti GR4]
ATGGCCACGAAATCGTCGACTGACACCCACGCGAAGCCCGTCAACCTGCGAATCCGAGAGGATATACGCAAGGTGATCGA
TCGCGCCGCGAGCATTCGCGGCAAGACCCGCTCCGATTTTATGATCGACGCAGCCTACCGCGCGGCCGAAGACACGCTGC
TCGATCAGACGCTGGTGCGGGTCGATGAGCACAGCTATCGGCACTACCTAGACATTCTTGACCAGCCGCCAAGTGGCGAC
GGCTTCGAACGCCTGATGAATGCGCCGAAACCCTGGCAGTCCTGA

Structures

Toxin

• AlphaFold DB: H0G6D3

Antitoxin

• AlphaFold DB: H0G6D4