Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 381377..381874 Replicon chromosome
Accession NZ_LR130532
Organism Escherichia coli isolate ecoli019

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EJK95_RS01755 Protein ID WP_001173343.1
Coordinates 381701..381874 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EJK95_RS01750 Protein ID WP_000398619.1
Coordinates 381377..381673 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJK95_RS01730 378108..378827 - 720 WP_000611288.1 two-component system response regulator DcuR -
EJK95_RS01735 378824..380455 - 1632 WP_001216486.1 sensor histidine kinase -
EJK95_RS01740 380636..380866 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
EJK95_RS01745 380878..381150 + 273 WP_000405647.1 N-acetyltransferase -
EJK95_RS01750 381377..381673 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EJK95_RS01755 381701..381874 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EJK95_RS01760 381993..383510 - 1518 WP_001295087.1 lysine--tRNA ligase -
EJK95_RS01765 383747..385204 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T286214 WP_001173343.1 NZ_LR130532:381701-381874 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT286214 WP_000398619.1 NZ_LR130532:381377-381673 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References