Detailed information of TA system TA286202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 5678841..5679511
• Replicon: chromosome
• Accession: NZ_LR130528
• Organism: Pseudomonas aeruginosa isolate paerg000

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EJN10_RS26980
• Protein ID: WP_023114647.1
• Coordinates: 5678841..5679260 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EJN10_RS26985
• Protein ID: WP_023114648.1
• Coordinates: 5679257..5679511 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJN10_RS26950	5674794..5675252	+	459	WP_023114640.1	helix-turn-helix domain-containing protein	-
EJN10_RS26955	5675291..5675659	-	369	WP_023114641.1	conjugal transfer transcriptional regulator TraJ	-
EJN10_RS26960	5675662..5675946	-	285	WP_023114642.1	hypothetical protein	-
EJN10_RS26965	5675958..5676200	-	243	WP_124034060.1	type I toxin-antitoxin system ptaRNA1 family toxin	-
EJN10_RS26970	5676269..5677740	-	1472	Protein_5196	P-type conjugative transfer protein TrbL	-
EJN10_RS31110	5677742..5677900	-	159	WP_172605026.1	hypothetical protein	-
EJN10_RS26975	5677915..5678685	-	771	WP_023114646.1	P-type conjugative transfer protein TrbJ	-
EJN10_RS26980	5678841..5679260	-	420	WP_023114647.1	type II toxin-antitoxin system VapC family toxin	Toxin
EJN10_RS26985	5679257..5679511	-	255	WP_023114648.1	Arc family DNA-binding protein	Antitoxin
EJN10_RS26990	5680806..5681783	-	978	WP_031637437.1	plasmid replication	-
EJN10_RS26995	5681764..5682657	-	894	WP_031637438.1	helicase RepA family protein	-
EJN10_RS27000	5682660..5682884	-	225	WP_023114650.1	AlpA family phage regulatory protein	-
EJN10_RS27005	5682978..5683970	+	993	WP_023114651.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5675662..5685574	9912

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14925.16 Da
• Isoelectric Point: 5.1890

>T286202 WP_023114647.1 NZ_LR130528:c5679260-5678841 [Pseudomonas aeruginosa]
MIVLDTNVVSEAMKPEPNPAVRAWLNEQVVETLYLSSVTLAELLFGIGALPNGKRKKGLGEALDGLLELFGERVLMFDTE
AARHYAELAVKARTAGKGFPTPDGYIGAIAASKGFIVATRDTSPFEAAGLTVINPWNHQ

Nucleotide

• Length: 420 bp

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9508.87 Da
• Isoelectric Point: 6.4760

>AT286202 WP_023114648.1 NZ_LR130528:c5679511-5679257 [Pseudomonas aeruginosa]
MAILTVRNVPDEVHRALRLRAAEHGRSTEAEVREILESAVKSEKRIRMGDALAELGRRVGLTNDDFAVLDQVRDKVPAEP
MRFE

Nucleotide

• Length: 255 bp