28620

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-RHH
Location 1369156..1369730 Replicon chromosome
Accession NC_019845
Organism Sinorhizobium meliloti GR4

Toxin (Protein)


Gene name vapC Uniprot ID A0A8B3NEQ7
Locus tag C770_RS06425 Protein ID WP_015241132.1
Coordinates 1369156..1369521 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q92QN3
Locus tag C770_RS06430 Protein ID WP_010969155.1
Coordinates 1369515..1369730 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C770_RS06400 1364296..1365738 + 1443 WP_010969150.1 NADH-quinone oxidoreductase subunit NuoN -
C770_RS06405 1365735..1366502 + 768 WP_003531861.1 biotin--[acetyl-CoA-carboxylase] ligase -
C770_RS06410 1366530..1368197 + 1668 WP_010969151.1 ribonuclease J -
C770_RS06415 1368256..1368660 + 405 WP_010969152.1 methylmalonyl-CoA epimerase -
C770_RS06420 1368800..1369069 + 270 WP_014526683.1 DUF1467 family protein -
C770_RS06425 1369156..1369521 - 366 WP_015241132.1 type II toxin-antitoxin system VapC family toxin Toxin
C770_RS06430 1369515..1369730 - 216 WP_010969155.1 ribbon-helix-helix protein, CopG family Antitoxin
C770_RS06435 1369798..1370025 - 228 WP_017264047.1 hypothetical protein -
C770_RS06440 1370441..1371769 + 1329 WP_003531873.1 proline--tRNA ligase -
C770_RS06445 1371790..1373100 + 1311 WP_014529722.1 lipoprotein-releasing ABC transporter permease subunit -
C770_RS06450 1373118..1373804 + 687 WP_010969157.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-40)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13606.59 Da        Isoelectric Point: 5.1787

>T28620 WP_015241132.1 NC_019845:c1369521-1369156 [Sinorhizobium meliloti GR4]
MVGALFDTNILIDHLNAVPQAHKELDRFENRAISIITWMEVMIGADAELVEPTRRFLDGFETIALNDEIANRAVTLRRAH
RIKLPDAVIWATAQTAGRLLVTRNTKDFPADDPGIREPYAV

Download         Length: 366 bp

>T28620 NC_019845:c1369521-1369156 [Sinorhizobium meliloti GR4]
ATGGTAGGCGCACTGTTCGATACCAATATCCTTATCGATCATCTGAATGCGGTGCCTCAGGCGCACAAGGAACTTGACCG
TTTCGAAAACCGGGCCATCAGCATCATCACCTGGATGGAGGTGATGATTGGAGCGGACGCCGAACTGGTTGAGCCGACCC
GGCGCTTCCTCGACGGTTTCGAAACGATCGCCTTGAACGATGAGATCGCCAACCGTGCGGTGACATTGCGCAGGGCGCAC
CGCATCAAGCTACCCGACGCGGTGATCTGGGCAACGGCGCAGACTGCCGGTCGTCTGCTCGTGACGCGCAATACCAAGGA
CTTTCCAGCCGACGACCCGGGGATACGCGAACCCTACGCCGTGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 8381.51 Da        Isoelectric Point: 8.6052

>AT28620 WP_010969155.1 NC_019845:c1369730-1369515 [Sinorhizobium meliloti GR4]
MRALIDMNDTQVEALDTLAKRVRRSRAALIREAIDDYLNRHHREQIEDGFGLWGKRKVDGLAYQEKVRGEW

Download         Length: 216 bp

>AT28620 NC_019845:c1369730-1369515 [Sinorhizobium meliloti GR4]
ATGCGAGCCTTGATCGATATGAACGATACCCAGGTCGAAGCCCTAGATACCCTCGCCAAGCGGGTGCGCCGGTCCCGTGC
GGCTCTGATCCGCGAAGCGATCGACGACTATCTCAACCGTCATCATCGCGAGCAGATCGAAGATGGCTTCGGTTTGTGGG
GCAAGCGCAAAGTTGACGGTCTGGCTTATCAGGAGAAGGTGCGCGGCGAATGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3NEQ7

Antitoxin

Source ID Structure
AlphaFold DB Q92QN3

References