Detailed information of TA system TA028617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 653201..653778
• Replicon: chromosome
• Accession: NC_019845
• Organism: Sinorhizobium meliloti GR4

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q92S20
• Locus tag: C770_RS50000
• Protein ID: WP_010968707.1
• Coordinates: 653201..653464 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q92KN5
• Locus tag: C770_RS02990
• Protein ID: WP_010968708.1
• Coordinates: 653461..653778 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C770_RS02950	648530..648970	+	441	WP_010968702.1	DUF2948 family protein	-
C770_RS02955	649035..650339	+	1305	WP_010968703.1	histidinol dehydrogenase	-
C770_RS02960	650336..650812	+	477	WP_010968704.1	UPF0262 family protein	-
C770_RS02965	650826..651305	+	480	WP_010968705.1	low molecular weight phosphatase family protein	-
C770_RS02970	651463..651681	+	219	WP_004435948.1	translation initiation factor IF-1	-
C770_RS02975	651779..652399	+	621	WP_004435951.1	Maf-like protein	-
C770_RS02980	652404..652616	+	213	WP_010968706.1	DNA gyrase inhibitor YacG	-
C770_RS50000	653201..653464	+	264	WP_010968707.1	BrnT family toxin	Toxin
C770_RS02990	653461..653778	+	318	WP_010968708.1	BrnA antitoxin family protein	Antitoxin
C770_RS02995	653981..654445	-	465	WP_013844019.1	type II toxin-antitoxin system HicB family antitoxin	-
C770_RS50005	654442..654630	-	189	WP_013844020.1	hypothetical protein	-
C770_RS03000	654950..656050	+	1101	WP_010968710.1	hypothetical protein	-
C770_RS03005	656037..657119	+	1083	WP_010968711.1	nucleotidyltransferase family protein	-
C770_RS03010	657322..657633	+	312	WP_004435964.1	hypothetical protein	-
C770_RS03020	657913..658302	-	390	WP_013844021.1	DUF930 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-103)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9821.16 Da
• Isoelectric Point: 10.3413

>T28617 WP_010968707.1 NC_019845:653201-653464 [Sinorhizobium meliloti GR4]
MRIVWGEPKRRANLEKHGFDFADVSDLDWASALIEDSKPDTSGRKRLKAIGYFRDGTAAVIFATLGTEAISIISFRPASD
RERRRLP

Nucleotide

• Length: 264 bp

>T28617 NC_019845:653201-653464 [Sinorhizobium meliloti GR4]
ATGCGAATTGTGTGGGGCGAACCCAAACGGCGAGCCAACCTTGAGAAGCATGGTTTCGATTTTGCCGATGTAAGCGACCT
CGATTGGGCGAGTGCCCTCATAGAGGACAGCAAGCCAGATACTTCTGGCAGAAAGCGCCTGAAGGCTATCGGTTATTTTC
GTGACGGGACGGCAGCGGTCATTTTCGCGACACTCGGAACGGAAGCTATATCGATCATCAGCTTTCGTCCTGCAAGCGAC
AGAGAAAGAAGGAGGCTTCCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12197.83 Da
• Isoelectric Point: 10.0883

>AT28617 WP_010968708.1 NC_019845:653461-653778 [Sinorhizobium meliloti GR4]
MTTRTRKSKTFEPGHGYTKEEWDAVDFPELTDEELTNMRPARDVLPPEFFKAMEEHRRSRGRPSLEHPKKQVTLRLDEDV
IAKFRAGGKGWQSRMNEALRKAAGI

Nucleotide

• Length: 318 bp

>AT28617 NC_019845:653461-653778 [Sinorhizobium meliloti GR4]
ATGACTACCAGGACCCGAAAATCGAAAACGTTCGAGCCAGGTCACGGCTATACAAAAGAGGAATGGGATGCAGTCGATTT
TCCCGAGTTGACCGATGAAGAGCTTACCAACATGCGTCCCGCAAGAGATGTGCTGCCGCCTGAGTTTTTCAAAGCTATGG
AGGAGCATCGCAGATCGCGTGGGCGCCCCTCGCTGGAGCATCCGAAAAAGCAAGTCACCCTCCGGTTGGACGAAGACGTG
ATCGCTAAGTTTCGAGCTGGTGGCAAAGGCTGGCAGAGCCGGATGAATGAGGCGCTGCGAAAAGCTGCAGGTATATAG

Structures

Toxin

• AlphaFold DB: Q92S20

Antitoxin

• AlphaFold DB: Q92KN5