Detailed information of TA system TA028615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 481173..481810
• Replicon: chromosome
• Accession: NC_019842
• Organism: Bacillus amyloliquefaciens subsp. plantarum AS43.3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: B938_RS02425
• Protein ID: WP_003156187.1
• Coordinates: 481460..481810 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: B938_RS02420
• Protein ID: WP_003156188.1
• Coordinates: 481173..481454 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B938_RS02400	477538..478137	-	600	WP_015239195.1	rhomboid family intramembrane serine protease	-
B938_RS02405	478230..478595	+	366	WP_007410229.1	holo-ACP synthase	-
B938_RS02410	478760..479767	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
B938_RS02415	479884..481053	+	1170	WP_015239196.1	alanine racemase	-
B938_RS02420	481173..481454	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
B938_RS02425	481460..481810	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
B938_RS02430	481929..482750	+	822	WP_007410232.1	STAS domain-containing protein	-
B938_RS02435	482755..483120	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
B938_RS02440	483123..483524	+	402	WP_003156178.1	anti-sigma regulatory factor	-
B938_RS02445	483536..484543	+	1008	WP_015239197.1	PP2C family protein-serine/threonine phosphatase	-
B938_RS02450	484607..484936	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
B938_RS02455	484933..485415	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
B938_RS02460	485381..486169	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
B938_RS02465	486169..486771	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T28615 WP_003156187.1 NC_019842:481460-481810 [Bacillus velezensis AS43.3]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T28615 NC_019842:481460-481810 [Bacillus velezensis AS43.3]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT28615 WP_003156188.1 NC_019842:481173-481454 [Bacillus velezensis AS43.3]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT28615 NC_019842:481173-481454 [Bacillus velezensis AS43.3]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5