Detailed information of TA system TA028612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1003276..1003859
• Replicon: chromosome
• Accession: NC_019780
• Organism: Dactylococcopsis salina PCC 8305

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DACSA_RS05125
• Protein ID: WP_198007636.1
• Coordinates: 1003515..1003859 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K9YS03
• Locus tag: DACSA_RS05120
• Protein ID: WP_015228731.1
• Coordinates: 1003276..1003518 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DACSA_RS05095	999686..1001008	-	1323	WP_015228726.1	efflux RND transporter periplasmic adaptor subunit	-
DACSA_RS05100	1001161..1001664	+	504	WP_015228727.1	PadR family transcriptional regulator	-
DACSA_RS05105	1001672..1001866	-	195	WP_015228728.1	hypothetical protein	-
DACSA_RS05110	1002013..1002273	-	261	WP_015228729.1	hypothetical protein	-
DACSA_RS05115	1002289..1002939	-	651	WP_015228730.1	uracil phosphoribosyltransferase	-
DACSA_RS05120	1003276..1003518	+	243	WP_015228731.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DACSA_RS05125	1003515..1003859	+	345	WP_198007636.1	endoribonuclease MazF	Toxin
DACSA_RS05130	1004169..1005704	+	1536	WP_015228733.1	carotene isomerase	-
DACSA_RS05135	1005841..1006059	-	219	WP_015228734.1	hypothetical protein	-
DACSA_RS21315	1006126..1006278	-	153	WP_015228735.1	hypothetical protein	-
DACSA_RS05140	1006640..1007035	+	396	WP_041235696.1	30S ribosome-binding factor RbfA	-
DACSA_RS05145	1007035..1008606	+	1572	WP_015228737.1	beta-glucosidase-like glycosyl hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-110)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13056.14 Da
• Isoelectric Point: 8.0123

>T28612 WP_198007636.1 NC_019780:1003515-1003859 [Dactylococcopsis salina PCC 8305]
VTENNQYIPRRGDLVYLNFNPTKGHEQQGHRPAFVASPYLYNQRTSLALFMPITKKQKGYPFEIILPSQLMIQGVILADQ
VKCLDWKARGVRFIESVPESLIDEVQATIEPLLL

Nucleotide

• Length: 345 bp

>T28612 NC_019780:1003515-1003859 [Dactylococcopsis salina PCC 8305]
GTGACAGAGAATAATCAATATATTCCCCGTCGTGGGGATTTGGTTTATTTAAATTTCAATCCGACAAAGGGGCATGAACA
GCAAGGTCATCGACCTGCTTTTGTTGCTTCTCCTTATCTTTACAATCAACGAACCAGTTTAGCTTTGTTTATGCCAATTA
CAAAAAAACAGAAAGGGTATCCATTTGAGATTATTCTCCCCTCACAATTAATGATTCAAGGAGTAATCTTGGCGGATCAG
GTGAAATGTTTGGACTGGAAAGCTCGCGGAGTAAGATTTATTGAGTCAGTACCAGAGAGTTTGATTGATGAAGTACAAGC
AACAATAGAACCTTTGCTACTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8870.01 Da
• Isoelectric Point: 6.7222

>AT28612 WP_015228731.1 NC_019780:1003276-1003518 [Dactylococcopsis salina PCC 8305]
MKGTVAKWGNSLVIRIPQHLAQQITLAEGTKVNFDVVDGNLVIKPRQRKHYSLEELTSGITSENLHNEVDTGTSVGNEVW

Nucleotide

• Length: 243 bp

>AT28612 NC_019780:1003276-1003518 [Dactylococcopsis salina PCC 8305]
ATGAAAGGAACTGTGGCGAAATGGGGTAACAGTCTTGTGATTCGGATTCCGCAACACTTAGCACAACAAATAACCTTAGC
TGAAGGAACAAAGGTCAACTTTGATGTAGTAGATGGAAATCTTGTCATCAAACCTAGACAGCGCAAGCATTATTCTCTGG
AAGAATTAACATCAGGCATTACATCGGAAAATCTTCATAATGAAGTAGATACAGGAACATCTGTGGGGAATGAAGTGTGG
TGA

Structures

Antitoxin

• AlphaFold DB: K9YS03