Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3116966..3117536 Replicon chromosome
Accession NZ_LR027516
Organism Mycobacterium tuberculosis strain DKC2 isolate Mycobacterium tuberculosis

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag DKC2_RS15345 Protein ID WP_003414166.1
Coordinates 3116966..3117322 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag DKC2_RS15350 Protein ID WP_003901465.1
Coordinates 3117306..3117536 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DKC2_RS15325 3112418..3114106 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
DKC2_RS15330 3114110..3114436 - 327 WP_003414157.1 hypothetical protein -
DKC2_RS15335 3114609..3115196 + 588 WP_003914429.1 DUF3558 family protein -
DKC2_RS15340 3115215..3116864 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
DKC2_RS15345 3116966..3117322 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
DKC2_RS15350 3117306..3117536 - 231 WP_003901465.1 antitoxin MazE Antitoxin
DKC2_RS15355 3117579..3118622 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
DKC2_RS15360 3118813..3119088 + 276 WP_003911993.1 DUF1778 domain-containing protein -
DKC2_RS21675 3119264..3119518 - 255 WP_003917684.1 hypothetical protein -
DKC2_RS15370 3119666..3120070 + 405 WP_009938577.1 hypothetical protein -
DKC2_RS15375 3120067..3120258 + 192 WP_003414184.1 hypothetical protein -
DKC2_RS15380 3120322..3121602 + 1281 Protein_2904 transposase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3116966..3129985 13019


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T285972 WP_003414166.1 NZ_LR027516:c3117322-3116966 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT285972 WP_003901465.1 NZ_LR027516:c3117536-3117306 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References