Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1700888..1701504 Replicon chromosome
Accession NZ_LR027516
Organism Mycobacterium tuberculosis strain DKC2 isolate Mycobacterium tuberculosis

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag DKC2_RS08285 Protein ID WP_003407593.1
Coordinates 1701187..1701504 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag DKC2_RS08280 Protein ID WP_003900349.1
Coordinates 1700888..1701190 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DKC2_RS08270 1696774..1698621 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
DKC2_RS08275 1698622..1700874 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
DKC2_RS08280 1700888..1701190 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
DKC2_RS08285 1701187..1701504 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
DKC2_RS08290 1701501..1702505 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
DKC2_RS08295 1702558..1703847 + 1290 WP_003407599.1 serine hydrolase -
DKC2_RS08300 1703920..1704693 - 774 WP_003911559.1 class I SAM-dependent methyltransferase -
DKC2_RS08305 1704751..1704963 - 213 WP_003898900.1 dodecin family protein -
DKC2_RS21610 1704973..1705197 - 225 WP_160522526.1 NUDIX domain-containing protein -
DKC2_RS08315 1705467..1706495 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T285948 WP_003407593.1 NZ_LR027516:1701187-1701504 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT285948 WP_003900349.1 NZ_LR027516:1700888-1701190 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References