Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1244757..1245388 Replicon chromosome
Accession NZ_LR027516
Organism Mycobacterium tuberculosis strain DKC2 isolate Mycobacterium tuberculosis

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag DKC2_RS06140 Protein ID WP_003898728.1
Coordinates 1244757..1245068 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag DKC2_RS06145 Protein ID WP_003405836.1
Coordinates 1245068..1245388 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DKC2_RS06120 1240238..1241662 - 1425 WP_030026248.1 class II fumarate hydratase -
DKC2_RS06125 1241693..1242781 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
DKC2_RS06130 1242837..1243481 + 645 WP_003915494.1 DUF4245 domain-containing protein -
DKC2_RS06135 1243488..1244645 - 1158 WP_003898727.1 AI-2E family transporter -
DKC2_RS06140 1244757..1245068 - 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
DKC2_RS06145 1245068..1245388 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
DKC2_RS06150 1245398..1246923 + 1526 Protein_1162 carboxylesterase/lipase family protein -
DKC2_RS06155 1246941..1248053 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
DKC2_RS06160 1248063..1248320 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
DKC2_RS06165 1248310..1249557 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
DKC2_RS06170 1249554..1250192 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T285942 WP_003898728.1 NZ_LR027516:c1245068-1244757 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT285942 WP_003405836.1 NZ_LR027516:c1245388-1245068 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References