Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2020459..2021243 Replicon chromosome
Accession NZ_LR025101
Organism Escherichia coli isolate EC-1639

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag EC1639_RS09530 Protein ID WP_000613626.1
Coordinates 2020459..2020953 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag EC1639_RS09535 Protein ID WP_001110447.1
Coordinates 2020950..2021243 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC1639_RS09510 2015652..2016749 + 1098 WP_000589326.1 flagellar basal body P-ring protein FlgI -
EC1639_RS09515 2016749..2017690 + 942 WP_001586904.1 flagellar assembly peptidoglycan hydrolase FlgJ -
EC1639_RS09520 2017756..2019399 + 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
EC1639_RS09525 2019411..2020364 + 954 WP_001586905.1 flagellar hook-associated protein FlgL -
EC1639_RS09530 2020459..2020953 - 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
EC1639_RS09535 2020950..2021243 - 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
EC1639_RS09540 2021376..2024561 - 3186 WP_001522867.1 ribonuclease E -
EC1639_RS09550 2025134..2026093 + 960 WP_001586906.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T285893 WP_000613626.1 NZ_LR025101:c2020953-2020459 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT285893 WP_001110447.1 NZ_LR025101:c2021243-2020950 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References