Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 751151..751727 Replicon chromosome
Accession NZ_LR025101
Organism Escherichia coli isolate EC-1639

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag EC1639_RS03540 Protein ID WP_001297643.1
Coordinates 751151..751438 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag EC1639_RS03545 Protein ID WP_000063156.1
Coordinates 751425..751727 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC1639_RS03520 747404..748684 - 1281 WP_001037380.1 DUF445 domain-containing protein -
EC1639_RS03525 748725..749957 - 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
EC1639_RS03530 750103..750198 - 96 Protein_653 dienelactone hydrolase family protein -
EC1639_RS03535 750255..750952 + 698 Protein_654 IS1 family transposase -
EC1639_RS03540 751151..751438 + 288 WP_001297643.1 BrnT family toxin Toxin
EC1639_RS03545 751425..751727 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
EC1639_RS03550 751761..752717 - 957 WP_001297640.1 GTPase -
EC1639_RS03555 752728..752931 - 204 WP_000467859.1 YbdD/YjiX family protein -
EC1639_RS03560 752981..755131 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T285887 WP_001297643.1 NZ_LR025101:751151-751438 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT285887 WP_000063156.1 NZ_LR025101:751425-751727 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References