Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 486048..486545 Replicon chromosome
Accession NZ_LR025101
Organism Escherichia coli isolate EC-1639

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EC1639_RS02225 Protein ID WP_001173343.1
Coordinates 486372..486545 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EC1639_RS02220 Protein ID WP_000398619.1
Coordinates 486048..486344 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC1639_RS02200 482777..483496 - 720 WP_000611288.1 two-component system response regulator DcuR -
EC1639_RS02205 483493..485124 - 1632 WP_001216477.1 sensor histidine kinase -
EC1639_RS02210 485305..485535 + 231 WP_000373291.1 (4Fe-4S)-binding protein -
EC1639_RS02215 485547..485819 + 273 WP_000405647.1 N-acetyltransferase -
EC1639_RS02220 486048..486344 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EC1639_RS02225 486372..486545 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EC1639_RS02230 486663..488180 - 1518 WP_000003806.1 lysine--tRNA ligase -
EC1639_RS02235 488417..489874 - 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T285883 WP_001173343.1 NZ_LR025101:486372-486545 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT285883 WP_000398619.1 NZ_LR025101:486048-486344 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References