Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 687516..688326 Replicon chromosome
Accession NZ_LR025099
Organism Klebsiella pneumoniae isolate EC-12536

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag EC12536_RS03380 Protein ID WP_004178461.1
Coordinates 687793..688326 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag EC12536_RS03375 Protein ID WP_002887278.1
Coordinates 687516..687782 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC12536_RS03355 683387..683731 - 345 WP_021441118.1 cation efflux system protein CusF -
EC12536_RS03360 683749..685134 - 1386 WP_048332967.1 efflux transporter outer membrane subunit -
EC12536_RS03365 685307..685990 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
EC12536_RS03370 685980..687413 + 1434 WP_134155573.1 Cu(+)/Ag(+) sensor histidine kinase -
EC12536_RS03375 687516..687782 + 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
EC12536_RS03380 687793..688326 + 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
EC12536_RS03385 688374..689495 - 1122 WP_021441121.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T285874 WP_004178461.1 NZ_LR025099:687793-688326 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT285874 WP_002887278.1 NZ_LR025099:687516-687782 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References