Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 38660..39285 Replicon plasmid 2
Accession NZ_LR025097
Organism Escherichia coli isolate EC-7215

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag EC7215_RS23710 Protein ID WP_000911324.1
Coordinates 38660..39058 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID L4J1D2
Locus tag EC7215_RS23715 Protein ID WP_000450532.1
Coordinates 39058..39285 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC7215_RS23695 34968..35489 + 522 WP_000632670.1 conjugal transfer entry exclusion protein TraS -
EC7215_RS23700 35514..36245 + 732 WP_000850422.1 complement resistance protein TraT -
EC7215_RS23705 36498..38651 + 2154 WP_000009350.1 type IV conjugative transfer system coupling protein TraD -
EC7215_RS23710 38660..39058 - 399 WP_000911324.1 tRNA(fMet)-specific endonuclease VapC Toxin
EC7215_RS23715 39058..39285 - 228 WP_000450532.1 toxin-antitoxin system antitoxin VapB Antitoxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 / mph(A) / blaTEM-1B / aadA2 / sul3 / ant(3'')-Ia / aph(3')-Ia / aph(3'')-Ib / aph(6)-Id / floR / tet(A) / sitABCD iroN / iroE / iroD / iroC / iroB 1..142200 142200


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14800.09 Da        Isoelectric Point: 7.8604

>T285869 WP_000911324.1 NZ_LR025097:c39058-38660 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMEVIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Download         Length: 399 bp

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8512.55 Da        Isoelectric Point: 4.5358

>AT285869 WP_000450532.1 NZ_LR025097:c39285-39058 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Download         Length: 228 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829CHF5

References