Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3957572..3958226 Replicon chromosome
Accession NZ_LR025096
Organism Escherichia coli isolate EC-7215

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag EC7215_RS19125 Protein ID WP_000244772.1
Coordinates 3957572..3957979 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EC7215_RS19130 Protein ID WP_000354046.1
Coordinates 3957960..3958226 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC7215_RS19105 3953529..3955262 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
EC7215_RS19110 3955268..3955978 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EC7215_RS19115 3956003..3956899 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EC7215_RS19120 3957011..3957532 + 522 WP_001055874.1 flavodoxin FldB -
EC7215_RS19125 3957572..3957979 - 408 WP_000244772.1 protein YgfX Toxin
EC7215_RS19130 3957960..3958226 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EC7215_RS19135 3958469..3959449 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EC7215_RS19145 3959702..3960682 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
EC7215_RS19150 3960805..3961464 - 660 WP_000250274.1 hemolysin III family protein -
EC7215_RS19155 3961628..3961939 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3959702..3960682 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T285863 WP_000244772.1 NZ_LR025096:c3957979-3957572 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT285863 WP_000354046.1 NZ_LR025096:c3958226-3957960 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References