Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3783584..3784167 Replicon chromosome
Accession NZ_LR025096
Organism Escherichia coli isolate EC-7215

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag EC7215_RS18320 Protein ID WP_000254745.1
Coordinates 3783584..3783919 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag EC7215_RS18325 Protein ID WP_000581937.1
Coordinates 3783919..3784167 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC7215_RS18300 3779053..3779415 + 363 WP_000034928.1 hypothetical protein -
EC7215_RS18305 3779471..3780769 - 1299 WP_000036723.1 phosphopyruvate hydratase -
EC7215_RS18310 3780857..3782494 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
EC7215_RS18315 3782722..3783513 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
EC7215_RS18320 3783584..3783919 - 336 WP_000254745.1 endoribonuclease MazF Toxin
EC7215_RS18325 3783919..3784167 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
EC7215_RS18330 3784245..3786479 - 2235 WP_027662018.1 GTP diphosphokinase -
EC7215_RS18335 3786527..3787828 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - vipA/tssB / vipB/tssC / hcp/tssD / clpV/tssH / tssF 3776652..3898428 121776


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T285862 WP_000254745.1 NZ_LR025096:c3783919-3783584 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT285862 WP_000581937.1 NZ_LR025096:c3784167-3783919 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References