285853

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1249321..1250158 Replicon chromosome
Accession NZ_LR025096
Organism Escherichia coli isolate EC-7215

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag EC7215_RS05870 Protein ID WP_000227784.1
Coordinates 1249321..1249863 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag EC7215_RS05875 Protein ID WP_001297137.1
Coordinates 1249847..1250158 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC7215_RS05850 1244860..1245771 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
EC7215_RS05855 1245939..1246430 + 492 WP_001138904.1 nucleotide binding protein YajQ -
EC7215_RS05860 1246558..1247922 - 1365 WP_001000978.1 MFS transporter -
EC7215_RS05865 1248330..1249265 + 936 WP_001297127.1 sel1 repeat family protein -
EC7215_RS05870 1249321..1249863 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
EC7215_RS05875 1249847..1250158 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
EC7215_RS05880 1250343..1251233 - 891 WP_000971336.1 heme o synthase -
EC7215_RS05885 1251245..1251574 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
EC7215_RS05890 1251574..1252188 - 615 WP_000179814.1 cytochrome o ubiquinol oxidase subunit III -
EC7215_RS05895 1252178..1254169 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
EC7215_RS05900 1254191..1255138 - 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T285853 WP_000227784.1 NZ_LR025096:c1249863-1249321 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT285853 WP_001297137.1 NZ_LR025096:c1250158-1249847 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References