Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 480035..480606 Replicon chromosome
Accession NZ_LN999844
Organism Enterococcus faecium isolate EFE10021

Toxin (Protein)


Gene name mazF Uniprot ID S4EH17
Locus tag EFE1002_RS02245 Protein ID WP_002306003.1
Coordinates 480265..480606 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag EFE1002_RS02240 Protein ID WP_002323011.1
Coordinates 480035..480265 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EFE1002_RS02215 475169..476500 + 1332 WP_002331664.1 FAD-containing oxidoreductase -
EFE1002_RS02220 476522..477148 + 627 WP_002286816.1 cysteine hydrolase -
EFE1002_RS02225 477331..477912 + 582 WP_002331663.1 TetR/AcrR family transcriptional regulator -
EFE1002_RS02230 478644..479219 + 576 WP_002331662.1 SOS response-associated peptidase family protein -
EFE1002_RS02235 479410..479748 - 339 WP_002306002.1 hypothetical protein -
EFE1002_RS02240 480035..480265 + 231 WP_002323011.1 hypothetical protein Antitoxin
EFE1002_RS02245 480265..480606 + 342 WP_002306003.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EFE1002_RS02250 481456..481641 + 186 WP_002304207.1 hypothetical protein -
EFE1002_RS02255 482146..482340 + 195 WP_002295146.1 hypothetical protein -
EFE1002_RS12710 482531..482780 + 250 Protein_452 transposase -
EFE1002_RS02265 483024..483854 - 831 WP_002286789.1 manganese catalase family protein -
EFE1002_RS02270 484062..485396 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13229.67 Da        Isoelectric Point: 10.3190

>T285811 WP_002306003.1 NZ_LN999844:480265-480606 [Enterococcus faecium]
VSGERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKKRKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT285811 WP_002323011.1 NZ_LN999844:480035-480265 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S4EH17

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References