Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 464201..464838 Replicon chromosome
Accession NZ_LN999829
Organism Bacillus velezensis strain B25

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BAMMD1_RS02295 Protein ID WP_003156187.1
Coordinates 464488..464838 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag BAMMD1_RS02290 Protein ID WP_003156188.1
Coordinates 464201..464482 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAMMD1_RS02270 460566..461165 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
BAMMD1_RS02275 461258..461623 + 366 WP_057080627.1 holo-ACP synthase -
BAMMD1_RS02280 461788..462795 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
BAMMD1_RS02285 462912..464081 + 1170 WP_003156189.1 alanine racemase -
BAMMD1_RS02290 464201..464482 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BAMMD1_RS02295 464488..464838 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BAMMD1_RS02300 464956..465777 + 822 WP_003156182.1 STAS domain-containing protein -
BAMMD1_RS02305 465782..466147 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
BAMMD1_RS02310 466150..466551 + 402 WP_003156178.1 anti-sigma regulatory factor -
BAMMD1_RS02315 466563..467570 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
BAMMD1_RS02320 467634..467963 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
BAMMD1_RS02325 467960..468442 + 483 WP_003156173.1 anti-sigma B factor RsbW -
BAMMD1_RS02330 468408..469196 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
BAMMD1_RS02335 469196..469798 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T285808 WP_003156187.1 NZ_LN999829:464488-464838 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT285808 WP_003156188.1 NZ_LN999829:464201-464482 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References