Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2714702..2715273 Replicon chromosome
Accession NC_019770
Organism Enterococcus faecalis str. Symbioflor 1

Toxin (Protein)


Gene name mazF Uniprot ID R3GRA7
Locus tag EFS1_RS13585 Protein ID WP_002360937.1
Coordinates 2714702..2715043 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S4CGQ1
Locus tag EFS1_RS13590 Protein ID WP_002367500.1
Coordinates 2715043..2715273 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EFS1_RS13580 2710717..2714331 - 3615 WP_002389470.1 DNA-directed RNA polymerase subunit beta -
EFS1_RS13585 2714702..2715043 - 342 WP_002360937.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EFS1_RS13590 2715043..2715273 - 231 WP_002367500.1 hypothetical protein Antitoxin
EFS1_RS14715 2715328..2715507 - 180 WP_111997057.1 hypothetical protein -
EFS1_RS13600 2715687..2715902 - 216 WP_002354768.1 zinc ribbon domain-containing protein -
EFS1_RS13605 2716041..2717033 + 993 WP_002389493.1 biotin--[acetyl-CoA-carboxylase] ligase -
EFS1_RS13610 2717297..2717935 - 639 WP_015212268.1 lytic polysaccharide monooxygenase -
EFS1_RS13620 2718621..2720237 + 1617 WP_015212269.1 phosphatase PAP2/LCP family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13165.41 Da        Isoelectric Point: 9.3988

>T28579 WP_002360937.1 NC_019770:c2715043-2714702 [Enterococcus faecalis str. Symbioflor 1]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T28579 NC_019770:c2715043-2714702 [Enterococcus faecalis str. Symbioflor 1]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGGCAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8704.69 Da        Isoelectric Point: 4.3313

>AT28579 WP_002367500.1 NC_019770:c2715273-2715043 [Enterococcus faecalis str. Symbioflor 1]
MLTTKSRRQGNSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Download         Length: 231 bp

>AT28579 NC_019770:c2715273-2715043 [Enterococcus faecalis str. Symbioflor 1]
ATGCTAACAACTAAATCAAGAAGACAAGGAAACTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A7G9

Antitoxin

Source ID Structure
AlphaFold DB S4CGQ1

References