Detailed information of TA system TA285736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1589599..1591294
• Replicon: chromosome
• Accession: NZ_LN871187
• Organism: Pseudomonas aeruginosa strain PAO1_Orsay

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: PAO1OR_RS07360
• Protein ID: WP_003112901.1
• Coordinates: 1589599..1590468 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: PAO1OR_RS07365
• Protein ID: WP_003112902.1
• Coordinates: 1590461..1591294 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAO1OR_RS07335	1585607..1586458	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
PAO1OR_RS07340	1586499..1587506	+	1008	WP_003112897.1	ABC transporter substrate-binding protein	-
PAO1OR_RS07345	1587503..1588279	+	777	WP_003112898.1	ABC transporter permease	-
PAO1OR_RS07350	1588285..1589046	+	762	WP_003112899.1	SDR family oxidoreductase	-
PAO1OR_RS07355	1589060..1589590	+	531	WP_003112900.1	cupin domain-containing protein	-
PAO1OR_RS07360	1589599..1590468	+	870	WP_003112901.1	alpha/beta hydrolase	Toxin
PAO1OR_RS07365	1590461..1591294	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
PAO1OR_RS07370	1591281..1592078	+	798	WP_003112903.1	SDR family oxidoreductase	-
PAO1OR_RS07375	1592071..1593753	+	1683	WP_003112904.1	thiamine pyrophosphate-binding protein	-
PAO1OR_RS07380	1593764..1594567	+	804	WP_003112905.1	aspartate dehydrogenase	-
PAO1OR_RS07385	1594579..1596063	+	1485	WP_003112906.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T285736 WP_003112901.1 NZ_LN871187:1589599-1590468 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT285736 WP_003112902.1 NZ_LN871187:1590461-1591294 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1