Detailed information of TA system TA028570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 383800..384730
• Replicon: chromosome
• Accession: NC_019770
• Organism: Enterococcus faecalis str. Symbioflor 1

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: EFS1_RS01975
• Protein ID: WP_002389521.1
• Coordinates: 383800..384456 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: EFS1_RS01980
• Protein ID: WP_000301765.1
• Coordinates: 384458..384730 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFS1_RS01935	378890..379120	-	231	WP_002355366.1	helix-turn-helix domain-containing protein	-
EFS1_RS01950	379976..380221	-	246	WP_002358448.1	helix-turn-helix domain-containing protein	-
EFS1_RS01955	380215..380616	-	402	WP_002364925.1	sigma-70 family RNA polymerase sigma factor	-
EFS1_RS01975	383800..384456	-	657	WP_002389521.1	zeta toxin family protein	Toxin
EFS1_RS01980	384458..384730	-	273	WP_000301765.1	antitoxin	Antitoxin
EFS1_RS01985	384747..384956	-	210	WP_002389526.1	peptide-binding protein	-
EFS1_RS01990	385026..385922	-	897	WP_096001398.1	ParA family protein	-
EFS1_RS01995	386311..387594	-	1284	WP_002363033.1	PTS sugar transporter subunit IIC	-
EFS1_RS02000	387611..388537	-	927	WP_002389507.1	hypothetical protein	-
EFS1_RS02005	388714..389442	+	729	WP_002389508.1	GntR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ClpL	EF0485 / bsh	379266..479894	100628

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 24481.80 Da
• Isoelectric Point: 6.1232

>T28570 WP_002389521.1 NC_019770:c384456-383800 [Enterococcus faecalis str. Symbioflor 1]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDKGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLLSDIRLYNREGVKLYSSLETG

Nucleotide

• Length: 657 bp

>T28570 NC_019770:c384456-383800 [Enterococcus faecalis str. Symbioflor 1]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAATTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATAAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCTAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTACTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACTGGATAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10689.19 Da
• Isoelectric Point: 6.4676

>AT28570 WP_000301765.1 NC_019770:c384730-384458 [Enterococcus faecalis str. Symbioflor 1]
VAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT28570 NC_019770:c384730-384458 [Enterococcus faecalis str. Symbioflor 1]
GTGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3