Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3036719..3037373 Replicon chromosome
Accession NZ_LN832404
Organism Escherichia coli K-12 substr. AG100

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag EK12AG100_RS15080 Protein ID WP_000244777.1
Coordinates 3036719..3037126 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EK12AG100_RS15085 Protein ID WP_000354046.1
Coordinates 3037107..3037373 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EK12AG100_RS15060 3032676..3034409 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
EK12AG100_RS15065 3034415..3035125 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EK12AG100_RS15070 3035150..3036046 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EK12AG100_RS15075 3036158..3036679 + 522 WP_001055874.1 flavodoxin FldB -
EK12AG100_RS15080 3036719..3037126 - 408 WP_000244777.1 toxin CptA Toxin
EK12AG100_RS15085 3037107..3037373 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EK12AG100_RS15090 3037616..3038596 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EK12AG100_RS15095 3038792..3039451 - 660 WP_000250274.1 hemolysin III family protein -
EK12AG100_RS15100 3039615..3039926 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
EK12AG100_RS15105 3039971..3041404 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
EK12AG100_RS15110 3041461..3042204 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T285667 WP_000244777.1 NZ_LN832404:c3037126-3036719 [Escherichia coli K-12]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT285667 WP_000354046.1 NZ_LN832404:c3037373-3037107 [Escherichia coli K-12]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References