Detailed information of TA system TA028566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 92064..92682
• Replicon: plasmid pSTA7437.01
• Accession: NC_019765
• Organism: Stanieria cyanosphaera PCC 7437

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K9XZS7
• Locus tag: STA7437_RS22515
• Protein ID: WP_015211942.1
• Coordinates: 92335..92682 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K9Y216
• Locus tag: STA7437_RS22510
• Protein ID: WP_015211941.1
• Coordinates: 92064..92330 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
STA7437_RS22500	88774..89706	+	933	WP_015211939.1	hypothetical protein	-
STA7437_RS22505	90045..91970	+	1926	WP_015211940.1	DNA-directed DNA polymerase	-
STA7437_RS22510	92064..92330	+	267	WP_015211941.1	CopG family protein	Antitoxin
STA7437_RS22515	92335..92682	+	348	WP_015211942.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
STA7437_RS25175	92709..93722	-	1014	WP_150109185.1	hypothetical protein	-
STA7437_RS22525	93813..94046	+	234	WP_041620436.1	hypothetical protein	-
STA7437_RS22530	94274..94930	-	657	WP_015211945.1	hypothetical protein	-
STA7437_RS22535	95020..95361	-	342	WP_015211946.1	hypothetical protein	-
STA7437_RS22540	95402..97150	-	1749	WP_015211947.1	VWA domain-containing protein	-
STA7437_RS22545	97280..97636	-	357	WP_015211948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..309296	309296

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13178.45 Da
• Isoelectric Point: 10.6786

>T28566 WP_015211942.1 NC_019765:92335-92682 [Stanieria cyanosphaera PCC 7437]
MSSPVYRQLEIRWIDLEPTRGAETQKKRPCVIIQADIVNQGSKTIIIAPILPGHKNWPFAVNVKPSKSNGLDKERHINLK
QMRAVDVSRIQNRQGILEQQYLKPLQEAIKIIFAL

Nucleotide

• Length: 348 bp

>T28566 NC_019765:92335-92682 [Stanieria cyanosphaera PCC 7437]
ATGAGTTCTCCTGTTTATCGGCAATTGGAAATAAGATGGATCGATCTCGAGCCGACAAGGGGTGCTGAAACTCAAAAAAA
AAGACCATGCGTTATTATCCAAGCCGACATTGTTAACCAGGGGTCGAAGACAATTATTATTGCTCCTATTCTTCCAGGAC
ATAAAAATTGGCCTTTTGCAGTCAATGTCAAACCTAGCAAGAGTAACGGATTAGATAAAGAACGACATATCAACCTCAAA
CAGATGAGAGCAGTTGATGTTTCCCGCATCCAAAATCGCCAAGGAATTCTGGAACAGCAATATTTGAAACCGCTTCAGGA
AGCTATTAAGATAATTTTCGCGCTCTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9891.02 Da
• Isoelectric Point: 4.2577

>AT28566 WP_015211941.1 NC_019765:92064-92330 [Stanieria cyanosphaera PCC 7437]
MTHRATITLDDDAYAFLEASAGNNRSAYINKLLKEEKQRVLAKQIMRANQEEASDLAYQEELMNWDVTLSDGLNERDTAV
SAACPIEL

Nucleotide

• Length: 267 bp

>AT28566 NC_019765:92064-92330 [Stanieria cyanosphaera PCC 7437]
ATGACCCATCGTGCAACTATTACTCTAGATGATGATGCCTACGCCTTTTTAGAAGCTTCAGCGGGGAATAATCGTAGTGC
TTATATCAATAAGCTGCTTAAAGAAGAAAAACAACGAGTATTGGCAAAACAGATAATGAGAGCCAATCAAGAAGAAGCTT
CTGACCTTGCTTATCAAGAAGAGCTAATGAATTGGGATGTAACATTGTCTGATGGATTGAACGAGAGGGACACTGCGGTT
TCCGCAGCTTGTCCAATCGAGTTATAA

Structures

Toxin

• AlphaFold DB: K9XZS7

Antitoxin

• AlphaFold DB: K9Y216