Detailed information of TA system TA028565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 9158..9942
• Replicon: plasmid pMIC7113.02
• Accession: NC_019760
• Organism: Microcoleus sp. PCC 7113

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: K9WRD4
• Locus tag: MIC7113_RS31395
• Protein ID: WP_015211486.1
• Coordinates: 9158..9655 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: K9WPK5
• Locus tag: MIC7113_RS31400
• Protein ID: WP_015211487.1
• Coordinates: 9652..9942 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MIC7113_RS31365	4452..5006	-	555	WP_015211481.1	hypothetical protein	-
MIC7113_RS31370	5444..6526	+	1083	WP_015185773.1	photosystem II q(b) protein	-
MIC7113_RS31375	6772..7041	-	270	WP_015211482.1	Txe/YoeB family addiction module toxin	-
MIC7113_RS31380	7034..7288	-	255	WP_015211483.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
MIC7113_RS31385	7355..8563	-	1209	WP_015211484.1	hypothetical protein	-
MIC7113_RS31390	8751..9095	-	345	WP_155898306.1	hypothetical protein	-
MIC7113_RS31395	9158..9655	-	498	WP_015211486.1	GNAT family N-acetyltransferase	Toxin
MIC7113_RS31400	9652..9942	-	291	WP_015211487.1	DUF1778 domain-containing protein	Antitoxin
MIC7113_RS31405	10319..12646	-	2328	WP_155898310.1	tetratricopeptide repeat protein	-
MIC7113_RS34070	12646..14832	-	2187	WP_051056019.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93032	93032

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (17-90)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18180.09 Da
• Isoelectric Point: 8.8020

>T28565 WP_015211486.1 NC_019760:c9655-9158 [Microcoleus sp. PCC 7113]
MSYTPPEPIKPTHDVETFDCGNPTLNDWLRKRAKTSETSGASRTYVICYENKVVGYYCLATGSVIRNDAPGKIRRNMPEP
IPVMVLGRLAVDINHQNKGIGKGLVKDAVMRTLQASEIAGIRAILVHALDEEAKQFYVERCGFSPSPVHPLTLMVLLADV
KKSLG

Nucleotide

• Length: 498 bp

>T28565 NC_019760:c9655-9158 [Microcoleus sp. PCC 7113]
ATGAGTTACACCCCACCAGAGCCGATAAAGCCGACACACGATGTAGAAACCTTCGATTGTGGGAACCCAACGCTAAATGA
CTGGTTGAGGAAACGCGCTAAGACGAGTGAAACCAGCGGAGCCTCGCGGACATACGTTATCTGCTATGAGAATAAAGTAG
TGGGGTACTACTGCTTAGCTACTGGTAGTGTCATTCGTAATGATGCTCCGGGCAAAATCAGACGGAATATGCCTGAGCCA
ATTCCGGTTATGGTATTGGGCAGACTTGCTGTTGACATTAACCACCAAAACAAAGGTATTGGTAAAGGTTTAGTCAAGGA
TGCTGTGATGCGTACTTTACAGGCATCAGAGATTGCGGGGATTAGAGCGATTCTGGTTCACGCCTTGGATGAAGAGGCAA
AACAATTCTATGTGGAGCGGTGTGGCTTTTCCCCCTCTCCAGTTCATCCCTTGACGCTCATGGTGCTGCTGGCAGATGTG
AAAAAGAGTCTTGGTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10898.35 Da
• Isoelectric Point: 4.8010

>AT28565 WP_015211487.1 NC_019760:c9942-9652 [Microcoleus sp. PCC 7113]
MAAPDRTQKRQRSEVVNMRIEPNQLDLIDTAANLCGKSRSAFMLDAAYRAAQEAMLDRRLFCLSDEQWEAFNKAIDTPPT
KNETLTQLLAAQAPWE

Nucleotide

• Length: 291 bp

>AT28565 NC_019760:c9942-9652 [Microcoleus sp. PCC 7113]
ATGGCAGCACCTGATAGAACCCAAAAGCGTCAGCGCTCCGAAGTCGTGAATATGAGAATTGAGCCAAATCAGCTCGACCT
CATCGATACGGCAGCGAATCTTTGTGGCAAGAGTCGGAGTGCCTTCATGCTAGATGCGGCGTATCGAGCAGCACAGGAAG
CAATGCTGGATCGGAGATTGTTCTGTCTGAGCGATGAGCAGTGGGAAGCCTTTAACAAAGCGATCGACACTCCGCCCACG
AAAAATGAAACGTTAACTCAGTTGCTTGCTGCACAAGCTCCGTGGGAATGA

Structures

Toxin

• AlphaFold DB: K9WRD4

Antitoxin

• AlphaFold DB: K9WPK5