Detailed information of TA system TA028564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 6772..7288
• Replicon: plasmid pMIC7113.02
• Accession: NC_019760
• Organism: Microcoleus sp. PCC 7113

Toxin (Protein)

• Gene name: relB
• Uniprot ID: K9WPK0
• Locus tag: MIC7113_RS31375
• Protein ID: WP_015211482.1
• Coordinates: 6772..7041 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: K9WQP9
• Locus tag: MIC7113_RS31380
• Protein ID: WP_015211483.1
• Coordinates: 7034..7288 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MIC7113_RS31350	2535..2744	-	210	WP_015211478.1	hypothetical protein	-
MIC7113_RS31355	2924..3811	-	888	WP_015211479.1	tyrosine-type recombinase/integrase	-
MIC7113_RS31360	4019..4318	+	300	WP_015211480.1	2Fe-2S iron-sulfur cluster binding domain-containing protein	-
MIC7113_RS31365	4452..5006	-	555	WP_015211481.1	hypothetical protein	-
MIC7113_RS31370	5444..6526	+	1083	WP_015185773.1	photosystem II q(b) protein	-
MIC7113_RS31375	6772..7041	-	270	WP_015211482.1	Txe/YoeB family addiction module toxin	Toxin
MIC7113_RS31380	7034..7288	-	255	WP_015211483.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
MIC7113_RS31385	7355..8563	-	1209	WP_015211484.1	hypothetical protein	-
MIC7113_RS31390	8751..9095	-	345	WP_155898306.1	hypothetical protein	-
MIC7113_RS31395	9158..9655	-	498	WP_015211486.1	GNAT family N-acetyltransferase	-
MIC7113_RS31400	9652..9942	-	291	WP_015211487.1	DUF1778 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..93032	93032

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10761.27 Da
• Isoelectric Point: 8.8001

>T28564 WP_015211482.1 NC_019760:c7041-6772 [Microcoleus sp. PCC 7113]
MSERKLAWTDEAWKDYIYWQSQDKKTLKRINKLIKETKSLPFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDDYLTI
ISCRYHYQL

Nucleotide

• Length: 270 bp

>T28564 NC_019760:c7041-6772 [Microcoleus sp. PCC 7113]
ATGAGTGAACGAAAATTAGCCTGGACTGATGAGGCTTGGAAAGACTATATCTATTGGCAAAGTCAAGATAAAAAGACCCT
GAAAAGAATTAATAAACTCATCAAGGAAACTAAAAGTCTTCCCTTCGAGGGAATTGGTAAACCTGAACCTTTGAAAGAAA
ATCTATCAGGGTTTTGGTCACGTCGTATTGATGATACTAATCGTCTTGTCTATGCGGTAGATGATGATTATTTAACGATT
ATTTCTTGTCGCTATCATTATCAGCTTTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9548.81 Da
• Isoelectric Point: 4.5700

>AT28564 WP_015211483.1 NC_019760:c7288-7034 [Microcoleus sp. PCC 7113]
MRVVTFSEARNKLKAILDQVVNDADYTVITRRDADDAVVMSLEYFNSLLETVHLLKSPANAAHLERSIAQYKQGQLIEQD
LLDE

Nucleotide

• Length: 255 bp

>AT28564 NC_019760:c7288-7034 [Microcoleus sp. PCC 7113]
ATGAGAGTAGTCACCTTTAGCGAAGCAAGAAATAAGCTAAAAGCAATTTTAGATCAGGTGGTTAATGATGCTGACTACAC
TGTAATTACTAGGCGGGATGCTGATGACGCCGTCGTTATGTCCCTTGAGTATTTTAATAGTTTGCTCGAAACCGTTCATT
TATTGAAGTCTCCAGCTAATGCAGCTCATTTAGAACGCTCCATTGCCCAGTACAAACAAGGTCAGCTTATAGAGCAAGAC
TTATTGGATGAGTGA

Structures

Toxin

• AlphaFold DB: K9WPK0

Antitoxin

• AlphaFold DB: K9WQP9