Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 4528956..4529497 Replicon chromosome
Accession NZ_LN831039
Organism Mycolicibacterium smegmatis strain NCTC8159

Toxin (Protein)


Gene name mazF Uniprot ID A0A2U9PVI3
Locus tag AT701_RS21725 Protein ID WP_003895797.1
Coordinates 4529168..4529497 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0R0N3
Locus tag AT701_RS21720 Protein ID WP_011729843.1
Coordinates 4528956..4529165 (+) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AT701_RS21695 4524227..4524997 - 771 WP_011729837.1 SDR family oxidoreductase -
AT701_RS21700 4525027..4525935 - 909 WP_011729838.1 cupin domain-containing protein -
AT701_RS21705 4525965..4526885 - 921 WP_011729840.1 NADP-dependent oxidoreductase -
AT701_RS21710 4526975..4527166 - 192 Protein_4249 hypothetical protein -
AT701_RS21715 4527383..4528795 + 1413 Protein_4250 dihydrolipoyl dehydrogenase -
AT701_RS21720 4528956..4529165 + 210 WP_011729843.1 antitoxin MazE family protein Antitoxin
AT701_RS21725 4529168..4529497 + 330 WP_003895797.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AT701_RS21730 4529540..4530103 - 564 WP_014878038.1 TetR/AcrR family transcriptional regulator -
AT701_RS21735 4530209..4531144 + 936 WP_003895799.1 alpha/beta fold hydrolase -
AT701_RS21740 4531141..4532853 + 1713 WP_058126565.1 NAD(P)/FAD-dependent oxidoreductase -
AT701_RS21750 4533070..4533450 + 381 WP_174519608.1 phage holin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-104)

Antitoxin

Antitoxin MazE-like

(5-68)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11739.52 Da        Isoelectric Point: 10.4023

>T285639 WP_003895797.1 NZ_LN831039:4529168-4529497 [Mycolicibacterium smegmatis]
VRRGDIYTAAARGAYTGKPRPVLIIQDDRFDATASVTVVPFTTSDTDAPLLRIRIEPTPATGLSTVSSLMIDKVTTVPRS
SLTHRVGRLAETDMVRTDRALLVFLGIAG

Download         Length: 330 bp

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 8114.98 Da        Isoelectric Point: 6.2231

>AT285639 WP_011729843.1 NZ_LN831039:4528956-4529165 [Mycolicibacterium smegmatis]
MTPARDRVRRHRERLRRQGLRPVQIWVPDVNAPEFRREAHRQSELVAAGEHEAEDQAFVDAISVDWDDA

Download         Length: 210 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U9PVI3

Antitoxin

Source ID Structure
AlphaFold DB A0R0N3

References