Detailed information of TA system TA028563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 3999279..3999750
• Replicon: chromosome
• Accession: NC_019753
• Organism: Crinalium epipsammum PCC 9333

Toxin (Protein)

• Gene name: -
• Uniprot ID: K9W4L5
• Locus tag: CRI9333_RS17455
• Protein ID: WP_015204500.1
• Coordinates: 3999490..3999750 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: K9W3M5
• Locus tag: CRI9333_RS17450
• Protein ID: WP_015204499.1
• Coordinates: 3999279..3999500 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRI9333_RS17440	3995764..3996690	-	927	WP_015204497.1	TIGR01777 family oxidoreductase	-
CRI9333_RS17445	3996796..3998985	-	2190	WP_015204498.1	protein kinase	-
CRI9333_RS17450	3999279..3999500	-	222	WP_015204499.1	hypothetical protein	Antitoxin
CRI9333_RS17455	3999490..3999750	-	261	WP_015204500.1	BrnT family toxin	Toxin
CRI9333_RS17460	4000066..4001496	+	1431	WP_015204501.1	dihydrolipoyl dehydrogenase	-
CRI9333_RS17465	4001598..4002485	+	888	WP_015204502.1	indole-3-glycerol phosphate synthase TrpC	-
CRI9333_RS17470	4002724..4002969	+	246	WP_015204503.1	DUF5340 domain-containing protein	-
CRI9333_RS17475	4002991..4003953	+	963	WP_015204504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10050.20 Da
• Isoelectric Point: 5.7378

>T28563 WP_015204500.1 NC_019753:c3999750-3999490 [Crinalium epipsammum PCC 9333]
VQFEYDSAKSQNNKSKHGIDFEEAQLLWNDPLRVEIQARSTTELRSIVIGKIGDKHWSAIITYRGEAIRIISVRRSRENE
EEIYES

Nucleotide

• Length: 261 bp

>T28563 NC_019753:c3999750-3999490 [Crinalium epipsammum PCC 9333]
GTGCAGTTTGAATACGACTCCGCAAAAAGCCAGAACAATAAGTCAAAGCATGGAATTGATTTTGAAGAAGCACAACTGCT
TTGGAATGACCCTCTGCGAGTAGAGATACAAGCACGCTCAACTACAGAACTTCGCTCAATAGTGATTGGTAAGATAGGAG
ATAAGCATTGGTCAGCAATTATTACCTATCGAGGTGAAGCCATAAGGATCATTTCAGTACGAAGATCGCGGGAGAATGAG
GAGGAAATTTATGAAAGCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8628.70 Da
• Isoelectric Point: 4.3525

>AT28563 WP_015204499.1 NC_019753:c3999500-3999279 [Crinalium epipsammum PCC 9333]
MKAEEFDQKFDDGEDVLEYLDFSTLRRPGLETKQVNVDFPQWMVDALDREAQRLGLHRQAVIKFWIAERLDAS

Nucleotide

• Length: 222 bp

>AT28563 NC_019753:c3999500-3999279 [Crinalium epipsammum PCC 9333]
ATGAAAGCTGAAGAATTCGATCAGAAGTTTGATGATGGGGAAGATGTCTTGGAATATTTAGATTTTTCAACACTGCGTCG
CCCAGGGTTAGAAACTAAGCAGGTTAATGTTGATTTTCCTCAGTGGATGGTGGATGCGTTAGACAGGGAAGCTCAACGCT
TAGGTCTTCATCGTCAAGCTGTAATTAAATTTTGGATTGCGGAACGCTTGGATGCAAGTTAG

Structures

Toxin

• AlphaFold DB: K9W4L5

Antitoxin

• AlphaFold DB: K9W3M5