Detailed information of TA system TA028562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 3199712..3200219
• Replicon: chromosome
• Accession: NC_019753
• Organism: Crinalium epipsammum PCC 9333

Toxin (Protein)

• Gene name: relB
• Uniprot ID: K9W0H3
• Locus tag: CRI9333_RS14020
• Protein ID: WP_015203820.1
• Coordinates: 3199959..3200219 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: K9W2Q5
• Locus tag: CRI9333_RS14015
• Protein ID: WP_015203819.1
• Coordinates: 3199712..3199966 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRI9333_RS14000	3196120..3198210	+	2091	WP_015203816.1	glycosyltransferase	-
CRI9333_RS14005	3198217..3198555	+	339	WP_015203817.1	hypothetical protein	-
CRI9333_RS14010	3198597..3199631	+	1035	WP_015203818.1	hypothetical protein	-
CRI9333_RS14015	3199712..3199966	+	255	WP_015203819.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
CRI9333_RS14020	3199959..3200219	+	261	WP_015203820.1	Txe/YoeB family addiction module toxin	Toxin
CRI9333_RS14025	3200366..3201286	-	921	WP_015203821.1	NAD(+) kinase	-
CRI9333_RS24985	3201322..3203007	-	1686	WP_015203822.1	serine/threonine protein kinase	-
CRI9333_RS14035	3203295..3203906	+	612	WP_015203823.1	IS607 family transposase	-
CRI9333_RS14040	3203914..3205047	+	1134	WP_157462326.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (7-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10450.98 Da
• Isoelectric Point: 6.9760

>T28562 WP_015203820.1 NC_019753:3199959-3200219 [Crinalium epipsammum PCC 9333]
MNRMLAWTDEAWADYLYWQEQDKKTLKRINKLISETMRMPFEGIGKPEPLRENLAGFWSRRIDDTNRLVYVVDDAYLTII
SCRYHY

Nucleotide

• Length: 261 bp

>T28562 NC_019753:3199959-3200219 [Crinalium epipsammum PCC 9333]
ATGAATAGAATGTTAGCTTGGACAGATGAGGCTTGGGCTGATTATCTTTACTGGCAAGAGCAAGACAAGAAAACACTCAA
AAGAATCAATAAACTGATTTCGGAGACGATGCGGATGCCTTTTGAGGGTATAGGTAAGCCAGAGCCTCTGCGAGAAAATT
TAGCCGGATTTTGGTCGCGGCGAATTGATGATACAAATCGTTTGGTTTATGTAGTTGATGATGCTTATCTAACTATTATT
TCTTGTCGATATCATTATTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9594.98 Da
• Isoelectric Point: 5.8295

>AT28562 WP_015203819.1 NC_019753:3199712-3199966 [Crinalium epipsammum PCC 9333]
MKVISFSSARNSLKTVLDRVTEDADYTIITRRDADDAVVMSLEFFNSLMETVYLLKSPANAAHLERSIAQFKQKKVVERD
LLNE

Nucleotide

• Length: 255 bp

>AT28562 NC_019753:3199712-3199966 [Crinalium epipsammum PCC 9333]
ATGAAAGTTATCTCTTTTAGCTCGGCAAGAAATAGTCTGAAGACGGTTTTAGATCGGGTAACTGAAGATGCCGACTATAC
AATCATCACCCGACGAGATGCGGATGACGCGGTGGTGATGTCGCTAGAGTTTTTTAATAGTCTGATGGAGACTGTGTATT
TACTTAAGTCTCCAGCCAATGCAGCCCATTTAGAACGTTCTATTGCTCAGTTTAAGCAAAAAAAAGTGGTAGAACGAGAT
TTGCTGAATGAATAG

Structures

Toxin

• AlphaFold DB: K9W0H3

Antitoxin

• AlphaFold DB: K9W2Q5