Detailed information of TA system TA028557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3430733..3431316
• Replicon: chromosome
• Accession: NC_019751
• Organism: Calothrix sp. PCC 6303

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K9V2B0
• Locus tag: CAL6303_RS14195
• Protein ID: WP_015198494.1
• Coordinates: 3430733..3431080 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K9V3Q0
• Locus tag: CAL6303_RS14200
• Protein ID: WP_015198495.1
• Coordinates: 3431074..3431316 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAL6303_RS14165	3426080..3426466	-	387	WP_015198488.1	hypothetical protein	-
CAL6303_RS14170	3426732..3427916	-	1185	WP_015198489.1	aspartate aminotransferase	-
CAL6303_RS14175	3427913..3429103	-	1191	WP_015198490.1	iron-containing alcohol dehydrogenase family protein	-
CAL6303_RS14180	3429245..3429796	-	552	WP_015198491.1	Ycf51 family protein	-
CAL6303_RS14185	3429910..3430161	+	252	WP_015198492.1	hypothetical protein	-
CAL6303_RS14190	3430158..3430604	+	447	WP_015198493.1	PIN domain-containing protein	-
CAL6303_RS14195	3430733..3431080	-	348	WP_015198494.1	endoribonuclease MazF	Toxin
CAL6303_RS14200	3431074..3431316	-	243	WP_015198495.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CAL6303_RS14205	3431807..3434395	-	2589	WP_015198496.1	glycosyltransferase family 39 protein	-
CAL6303_RS14210	3434602..3435189	-	588	WP_015198497.1	Uma2 family endonuclease	-
CAL6303_RS14215	3435281..3436147	-	867	WP_015198498.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (12-111)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13156.30 Da
• Isoelectric Point: 6.9786

>T28557 WP_015198494.1 NC_019751:c3431080-3430733 [Calothrix sp. PCC 6303]
MVTPSNPYIPDRGDIVYLDFNPTKSREQRGHRPAFVLSPRSYNAKSSLALFMPITKQQKGYPFEVILPSELQIQGVILAD
QIKCLDWKVRDVQFVELVSKDIVEEVQAKIEPLIL

Nucleotide

• Length: 348 bp

>T28557 NC_019751:c3431080-3430733 [Calothrix sp. PCC 6303]
TTGGTAACACCATCAAATCCTTATATCCCAGACCGGGGAGATATCGTCTATCTGGATTTTAATCCAACCAAAAGTCGTGA
ACAAAGGGGGCATCGCCCTGCTTTTGTGCTTTCACCTCGTAGTTACAACGCAAAAAGTTCTCTGGCACTGTTTATGCCCA
TCACTAAACAGCAGAAAGGATATCCCTTTGAAGTTATTTTGCCGTCTGAGTTGCAGATTCAAGGTGTGATTCTTGCCGAT
CAAATCAAGTGTTTGGACTGGAAAGTCCGTGACGTTCAGTTTGTTGAATTGGTTTCAAAAGATATCGTTGAGGAAGTGCA
GGCAAAAATTGAACCGTTAATTTTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8920.30 Da
• Isoelectric Point: 4.7616

>AT28557 WP_015198495.1 NC_019751:c3431316-3431074 [Calothrix sp. PCC 6303]
MVATVAKWGNSLAIRLPQHLAKEIQLTEGVEVDLVVIDGNLVIKPRIRKRYSLDELISEITPENLHTEVESGIAMGNEAW

Nucleotide

• Length: 243 bp

>AT28557 NC_019751:c3431316-3431074 [Calothrix sp. PCC 6303]
ATGGTTGCGACAGTTGCAAAGTGGGGAAATAGTCTAGCCATTCGACTTCCCCAGCACTTAGCTAAAGAAATCCAGTTAAC
AGAAGGCGTTGAGGTTGATCTTGTGGTGATCGATGGCAATCTAGTCATCAAACCTAGAATCAGAAAGCGGTACTCCCTAG
ATGAACTGATTTCGGAAATCACGCCAGAAAATCTCCATACTGAGGTTGAGAGTGGGATTGCTATGGGGAATGAAGCTTGG
TAA

Structures

Toxin

• AlphaFold DB: K9V2B0

Antitoxin

• AlphaFold DB: K9V3Q0