Detailed information of TA system TA028556

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 2027327..2028213
• Replicon: chromosome
• Accession: NC_019751
• Organism: Calothrix sp. PCC 6303

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: -
• Locus tag: CAL6303_RS08275
• Protein ID: WP_015197388.1
• Coordinates: 2027770..2028213 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: K9V0G6
• Locus tag: CAL6303_RS08270
• Protein ID: WP_015197387.1
• Coordinates: 2027327..2027773 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAL6303_RS28810	2022782..2023600	-	819	Protein_1658	WYL domain-containing protein	-
CAL6303_RS08250	2023764..2024024	+	261	WP_015197383.1	DUF433 domain-containing protein	-
CAL6303_RS08255	2024021..2024386	+	366	WP_015197384.1	DUF5615 family PIN-like protein	-
CAL6303_RS08260	2024395..2026578	+	2184	WP_041740355.1	type I-D CRISPR-associated helicase Cas3'	-
CAL6303_RS08265	2026796..2027065	+	270	WP_015197386.1	hypothetical protein	-
CAL6303_RS29825	2027079..2027242	+	164	Protein_1663	DUF433 domain-containing protein	-
CAL6303_RS08270	2027327..2027773	+	447	WP_015197387.1	nucleotidyltransferase domain-containing protein	Antitoxin
CAL6303_RS08275	2027770..2028213	+	444	WP_015197388.1	DUF86 domain-containing protein	Toxin
CAL6303_RS08280	2028232..2029251	+	1020	WP_015197389.1	DUF1016 family protein	-
CAL6303_RS08285	2029276..2032632	+	3357	WP_015197390.1	type I-D CRISPR-associated protein Cas10d/Csc3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (21-133)

Antitoxin

• Polymerase beta, nucleotidyltransferase (15-111)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16987.45 Da
• Isoelectric Point: 4.7650

>T28556 WP_015197388.1 NC_019751:2027770-2028213 [Calothrix sp. PCC 6303]
MSKIDPEIVLARLRLITKYKNTLEEFRSININEFLAEFRQQLIVERLLQLMIQAAIDINDHILSRLNPGNSATNFEAFIE
LGKYEVITSELAKQLAPSGSLRNRLVHEYDDIDPNQVYQAIKFALQQYPLYVQQINAYLISLDTESS

Nucleotide

• Length: 444 bp

>T28556 NC_019751:2027770-2028213 [Calothrix sp. PCC 6303]
ATGAGTAAGATAGACCCAGAAATTGTGTTAGCTAGATTGAGATTAATCACAAAGTATAAAAATACTTTAGAGGAGTTTAG
GTCTATAAATATAAATGAGTTTTTAGCGGAATTTAGGCAACAGTTAATTGTCGAAAGACTTTTACAATTGATGATTCAAG
CCGCGATAGATATTAATGACCATATTTTATCAAGATTGAATCCTGGAAATTCAGCTACTAACTTTGAAGCATTTATCGAA
CTTGGTAAGTATGAGGTTATTACTTCGGAATTAGCAAAGCAACTTGCACCATCAGGAAGTTTAAGGAATCGCTTAGTTCA
TGAATATGATGACATAGACCCTAACCAGGTTTATCAAGCTATCAAATTTGCTTTACAACAATATCCTTTGTACGTACAAC
AAATAAATGCTTATCTTATTTCTCTTGATACGGAAAGTAGCTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 17241.92 Da
• Isoelectric Point: 6.0974

>AT28556 WP_015197387.1 NC_019751:2027327-2027773 [Calothrix sp. PCC 6303]
MNTPTIEKLKQLAQQIPEKIPYLKMLVLFGSRATGETHVNSDWDFAVLIDKAQQEIYTKDNAWGAFGLSMFIGEILNINP
DKIDIVELNHCSWLIAHFVARDGILIFEKESSGFEYFRLTSLRPESELKKFRKEQHQLIEMQLKQWSI

Nucleotide

• Length: 447 bp

>AT28556 NC_019751:2027327-2027773 [Calothrix sp. PCC 6303]
ATGAACACACCAACAATAGAAAAACTCAAACAACTTGCTCAACAAATTCCTGAGAAAATTCCTTATCTCAAAATGTTAGT
TTTATTTGGTTCGAGAGCAACAGGGGAAACCCACGTAAATAGTGATTGGGATTTTGCTGTTCTTATAGATAAAGCACAAC
AAGAGATTTATACAAAAGATAATGCTTGGGGTGCATTTGGACTATCAATGTTTATCGGAGAAATATTAAATATTAATCCT
GACAAAATTGATATTGTAGAACTCAATCATTGTTCGTGGTTAATTGCTCATTTTGTAGCGCGTGATGGCATTTTAATTTT
TGAAAAAGAATCATCAGGATTTGAATATTTTCGCCTCACGTCATTAAGACCTGAATCAGAATTAAAGAAATTCAGAAAAG
AACAACATCAACTTATTGAAATGCAACTTAAGCAGTGGTCAATATGA

Structures

Antitoxin

• AlphaFold DB: K9V0G6