Detailed information of TA system TA028555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 953728..954314
• Replicon: chromosome
• Accession: NC_019751
• Organism: Calothrix sp. PCC 6303

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: K9UWC0
• Locus tag: CAL6303_RS03930
• Protein ID: WP_015196530.1
• Coordinates: 953928..954314 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CAL6303_RS03925
• Protein ID: WP_041739065.1
• Coordinates: 953728..953931 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAL6303_RS03910	949349..950008	-	660	WP_015196526.1	ribosome-associated translation inhibitor RaiA	-
CAL6303_RS03915	950650..951294	+	645	WP_041739062.1	lipoyl(octanoyl) transferase LipB	-
CAL6303_RS03920	951307..953169	-	1863	WP_015196528.1	alkaline phosphatase family protein	-
CAL6303_RS03925	953728..953931	+	204	WP_041739065.1	hypothetical protein	Antitoxin
CAL6303_RS03930	953928..954314	+	387	WP_015196530.1	type II toxin-antitoxin system VapC family toxin	Toxin
CAL6303_RS03935	954377..955108	-	732	WP_015196531.1	DUF3239 domain-containing protein	-
CAL6303_RS03940	955223..956386	-	1164	WP_015196532.1	extracellular solute-binding protein	-
CAL6303_RS03945	956462..956740	-	279	WP_015196533.1	DUF2470 domain-containing protein	-
CAL6303_RS03950	957073..957795	+	723	WP_015196534.1	serine/threonine protein phosphatase	-
CAL6303_RS03955	957854..958714	-	861	WP_144051000.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14872.14 Da
• Isoelectric Point: 6.2191

>T28555 WP_015196530.1 NC_019751:953928-954314 [Calothrix sp. PCC 6303]
MKALLDTHTFLWWVTNNLQLSSTVKSILSERNNRIFFSAASSWEIAIKAQLSKLELPSNPEVYIAEQLEINSFQVLPIEL
KYTLQTYYLPNHHKDPFDRILVAQSQVENLPLLTLDPKIAQYQVSIIW

Nucleotide

• Length: 387 bp

>T28555 NC_019751:953928-954314 [Calothrix sp. PCC 6303]
ATGAAAGCACTTTTGGATACCCACACATTCCTCTGGTGGGTAACGAATAACCTGCAACTATCATCAACTGTAAAATCTAT
TTTGAGCGAACGTAATAATCGGATTTTTTTTAGTGCTGCGAGTTCTTGGGAAATTGCAATAAAAGCTCAACTCTCAAAGC
TAGAATTACCTAGTAATCCTGAAGTATATATTGCAGAACAACTAGAAATCAACTCATTTCAAGTTTTGCCGATTGAATTA
AAATATACTTTGCAAACTTATTATTTGCCCAATCATCACAAAGATCCTTTTGATAGAATTTTAGTTGCTCAAAGTCAAGT
AGAGAACCTTCCCCTACTAACCTTAGATCCAAAAATTGCTCAATATCAAGTTAGTATAATTTGGTAG

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7229.36 Da
• Isoelectric Point: 3.7455

>AT28555 WP_041739065.1 NC_019751:953728-953931 [Calothrix sp. PCC 6303]
INLLDLLDRVNQGEEITILKDGEQIARLVPPLPTLPPRVPGTAIGMITIAPDFDEPLPDDILAAFEG

Nucleotide

• Length: 204 bp

>AT28555 NC_019751:953728-953931 [Calothrix sp. PCC 6303]
ATCAACCTGCTTGATTTGCTAGATAGAGTTAATCAAGGTGAGGAGATTACCATTCTCAAAGATGGTGAGCAGATTGCTCG
ACTTGTTCCCCCATTGCCTACTTTACCACCCAGAGTTCCAGGTACCGCAATCGGAATGATTACCATCGCACCTGATTTTG
ATGAACCTTTACCAGATGATATCCTGGCAGCGTTTGAAGGATGA

Structures

Toxin

• AlphaFold DB: K9UWC0