Detailed information of TA system TA028553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TacT2-ataR/DUF1778(antitoxin)
• Location: 4290083..4290858
• Replicon: chromosome
• Accession: NC_019748
• Organism: Stanieria cyanosphaera PCC 7437

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: K9XZ17
• Locus tag: STA7437_RS18655
• Protein ID: WP_015194939.1
• Coordinates: 4290337..4290858 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: K9XXM9
• Locus tag: STA7437_RS18650
• Protein ID: WP_015194938.1
• Coordinates: 4290083..4290349 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
STA7437_RS18625	4285686..4286798	-	1113	Protein_3746	transposase	-
STA7437_RS18630	4286966..4287365	+	400	Protein_3747	endonuclease domain-containing protein	-
STA7437_RS18635	4287374..4287577	+	204	Protein_3748	hypothetical protein	-
STA7437_RS18640	4287678..4288918	-	1241	Protein_3749	IS200/IS605 family element transposase accessory protein TnpB	-
STA7437_RS18645	4288990..4290012	+	1023	Protein_3750	hypothetical protein	-
STA7437_RS18650	4290083..4290349	+	267	WP_015194938.1	DUF1778 domain-containing protein	Antitoxin
STA7437_RS18655	4290337..4290858	+	522	WP_015194939.1	GNAT family N-acetyltransferase	Toxin
STA7437_RS18660	4290855..4291250	-	396	WP_015194940.1	lecithin retinol acyltransferase family protein	-
STA7437_RS18665	4291418..4294813	+	3396	Protein_3754	ATP-binding protein	-
STA7437_RS18670	4295081..4295428	+	348	WP_015194941.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4288130..4288849	719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (47-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (11-81)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19406.74 Da
• Isoelectric Point: 10.2853

>T28553 WP_015194939.1 NC_019748:4290337-4290858 [Stanieria cyanosphaera PCC 7437]
MGRVKYSQPQRISREHIVENFDCGEISLNDWLKKRALKNDLGDASRTYVVCCNNTVIAYYSLHLGCIQHSEAIRKIKHNM
PDPIPAIVLGRLAVDKLHQGKGLARALIKDMFLRAIQVSDLAGTKAVLVKALNEKVTVFYQSFGFVQSKTDPLLLMKAIA
EVRASLNQKSKKN

Nucleotide

• Length: 522 bp

>T28553 NC_019748:4290337-4290858 [Stanieria cyanosphaera PCC 7437]
TTGGGAAGAGTAAAATATTCACAACCGCAACGTATTTCAAGAGAGCATATTGTAGAAAACTTTGATTGTGGTGAAATTTC
TCTCAACGATTGGCTGAAAAAACGCGCTCTTAAAAATGATCTGGGAGATGCTAGTAGAACTTACGTTGTCTGCTGCAATA
ATACAGTAATAGCTTATTACTCACTTCATTTAGGCTGTATTCAACATTCCGAAGCCATAAGAAAAATTAAGCACAATATG
CCCGATCCGATTCCAGCTATAGTTTTAGGTAGGCTAGCTGTTGATAAACTTCATCAAGGAAAAGGATTAGCCAGAGCTTT
GATTAAAGATATGTTCCTGAGAGCTATTCAAGTTTCTGATTTGGCTGGTACTAAAGCAGTTTTAGTTAAAGCTCTTAATG
AGAAGGTGACTGTTTTTTATCAATCCTTTGGTTTTGTTCAGTCAAAAACCGACCCTCTTTTGTTAATGAAAGCGATCGCT
GAAGTAAGAGCTAGTTTGAATCAGAAAAGTAAAAAAAATTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10309.85 Da
• Isoelectric Point: 5.9082

>AT28553 WP_015194938.1 NC_019748:4290083-4290349 [Stanieria cyanosphaera PCC 7437]
MATIEKQKVVNLRIDEVRRNLIDEAAKLQGKSRTEFMVEAAYKEAEQTILDQKIFILDEADCEYLSRTNHKPDPKLVKLF
KSKSPWEE

Nucleotide

• Length: 267 bp

>AT28553 NC_019748:4290083-4290349 [Stanieria cyanosphaera PCC 7437]
ATGGCAACGATAGAAAAACAAAAAGTAGTCAACCTGAGAATTGACGAAGTTAGGCGCAATTTAATCGATGAAGCAGCAAA
GCTTCAAGGTAAATCCCGTACTGAGTTTATGGTAGAAGCTGCTTACAAAGAAGCCGAACAAACTATCTTAGACCAGAAGA
TTTTCATTTTAGATGAAGCAGATTGTGAATATCTTTCTCGTACAAACCATAAACCAGACCCCAAATTAGTCAAATTGTTT
AAGAGCAAAAGCCCTTGGGAAGAGTAA

Structures

Toxin

• AlphaFold DB: K9XZ17

Antitoxin

• AlphaFold DB: K9XXM9