Detailed information of TA system TA028552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3957400..3957974
• Replicon: chromosome
• Accession: NC_019748
• Organism: Stanieria cyanosphaera PCC 7437

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K9XWN4
• Locus tag: STA7437_RS17225
• Protein ID: WP_015194667.1
• Coordinates: 3957636..3957974 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K9XY66
• Locus tag: STA7437_RS17220
• Protein ID: WP_015194666.1
• Coordinates: 3957400..3957642 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
STA7437_RS17200	3952822..3953808	-	987	WP_015194662.1	zinc-dependent alcohol dehydrogenase family protein	-
STA7437_RS17205	3954049..3954876	+	828	WP_015194663.1	alpha/beta hydrolase	-
STA7437_RS17210	3955124..3955609	+	486	WP_015194664.1	hypothetical protein	-
STA7437_RS17215	3955814..3957205	+	1392	WP_015194665.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
STA7437_RS17220	3957400..3957642	+	243	WP_015194666.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
STA7437_RS17225	3957636..3957974	+	339	WP_015194667.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
STA7437_RS17230	3958010..3958834	-	825	WP_015194668.1	fatty acid desaturase	-
STA7437_RS17235	3959064..3960293	+	1230	WP_015194669.1	TetR family transcriptional regulator	-
STA7437_RS17240	3960439..3961269	-	831	WP_015194670.1	CPBP family intramembrane metalloprotease	-
STA7437_RS17245	3961405..3961698	-	294	WP_015194671.1	ATP-dependent Clp protease adapter ClpS	-
STA7437_RS17250	3961940..3962857	+	918	WP_015194672.1	alpha/beta fold hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-108)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12599.80 Da
• Isoelectric Point: 9.5023

>T28552 WP_015194667.1 NC_019748:3957636-3957974 [Stanieria cyanosphaera PCC 7437]
MVKYYIPDRGDIIKLSFSPQQGKEQAGIRPALVISPVAYNRISHFILACPITSQVKGWRFEVVLPQGLNTYGVILADQVR
LLDWQIRQAKLIEKAPSEVIEEVLARLSPLVS

Nucleotide

• Length: 339 bp

>T28552 NC_019748:3957636-3957974 [Stanieria cyanosphaera PCC 7437]
ATGGTAAAGTATTACATCCCAGATAGAGGAGACATTATCAAACTTTCATTTAGTCCCCAACAAGGCAAAGAACAAGCAGG
AATAAGACCAGCATTAGTAATTTCCCCTGTTGCATATAATCGTATCTCTCATTTTATCCTAGCTTGTCCAATTACCAGCC
AGGTAAAAGGTTGGCGGTTTGAAGTTGTTTTACCCCAAGGATTAAATACTTATGGTGTTATTTTAGCCGACCAAGTGCGG
TTATTAGATTGGCAAATTCGTCAAGCAAAGTTGATCGAAAAAGCACCATCAGAAGTTATTGAAGAAGTATTAGCACGTTT
ATCTCCTTTAGTGTCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9140.44 Da
• Isoelectric Point: 4.6749

>AT28552 WP_015194666.1 NC_019748:3957400-3957642 [Stanieria cyanosphaera PCC 7437]
MPIKIAKWGNSLGIRIPKHLAEQVQLQEGDEIEISTAEDKLIVTPLKRKKYTLEQLLEGMGEENLHSEIDWGEATGKEQW

Nucleotide

• Length: 243 bp

>AT28552 NC_019748:3957400-3957642 [Stanieria cyanosphaera PCC 7437]
ATGCCTATTAAAATAGCTAAATGGGGTAATAGCTTGGGTATTCGTATCCCAAAACACTTAGCTGAACAAGTACAATTGCA
AGAAGGAGATGAAATAGAAATATCAACAGCAGAAGACAAACTAATTGTTACTCCTCTCAAGCGCAAGAAATATACTTTAG
AGCAATTACTCGAAGGAATGGGAGAAGAGAATTTGCACTCGGAAATAGATTGGGGTGAAGCGACGGGAAAAGAACAATGG
TAA

Structures

Toxin

• AlphaFold DB: K9XWN4

Antitoxin

• AlphaFold DB: K9XY66