Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 518642..519278 Replicon chromosome
Accession NZ_LN649259
Organism Bacillus subtilis strain BS49

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag VV34_RS02665 Protein ID WP_003156187.1
Coordinates 518928..519278 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag VV34_RS02660 Protein ID WP_003225183.1
Coordinates 518642..518923 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VV34_RS02640 515001..515600 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
VV34_RS02645 515695..516060 + 366 WP_003234281.1 holo-ACP synthase -
VV34_RS02650 516226..517242 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
VV34_RS02655 517357..518526 + 1170 WP_003234284.1 alanine racemase -
VV34_RS02660 518642..518923 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
VV34_RS02665 518928..519278 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
VV34_RS02670 519393..520217 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
VV34_RS02675 520222..520587 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
VV34_RS02680 520591..520992 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
VV34_RS02685 521004..522011 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
VV34_RS02690 522073..522402 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
VV34_RS02695 522399..522881 + 483 WP_003234299.1 anti-sigma B factor RsbW -
VV34_RS02700 522847..523635 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
VV34_RS02705 523635..524234 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T285500 WP_003156187.1 NZ_LN649259:518928-519278 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT285500 WP_003225183.1 NZ_LN649259:518642-518923 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References