Detailed information of TA system TA028548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 95560..96130
• Replicon: chromosome
• Accession: NC_019748
• Organism: Stanieria cyanosphaera PCC 7437

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K9XMI9
• Locus tag: STA7437_RS00415
• Protein ID: WP_015191381.1
• Coordinates: 95780..96130 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: K9XPV3
• Locus tag: STA7437_RS00410
• Protein ID: WP_015191380.1
• Coordinates: 95560..95799 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
STA7437_RS00390	91193..92176	+	984	WP_015191376.1	RNA polymerase sigma factor, RpoD/SigA family	-
STA7437_RS00395	92397..94046	+	1650	WP_015191377.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
STA7437_RS00400	94344..95177	-	834	WP_015191378.1	aldo/keto reductase	-
STA7437_RS00405	95248..95514	-	267	WP_015191379.1	hypothetical protein	-
STA7437_RS00410	95560..95799	+	240	WP_015191380.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
STA7437_RS00415	95780..96130	+	351	WP_015191381.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
STA7437_RS00420	96265..97533	-	1269	WP_015191382.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
STA7437_RS00425	97648..99195	-	1548	WP_015191383.1	B12-binding domain-containing radical SAM protein	-
STA7437_RS00430	99624..100130	+	507	WP_150109097.1	N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (13-113)

Antitoxin

• SpoVT-AbrB domain (6-48)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13075.45 Da
• Isoelectric Point: 10.3846

>T28548 WP_015191381.1 NC_019748:95780-96130 [Stanieria cyanosphaera PCC 7437]
VKKSGSKQYIPDRGDIIWLDFNPQIGREQAGHRPAIVISPKKFNSLSSLVFVCPISSKIKGFSFEVLLTEQMQTKGVVLI
HHLRSVDWKTRGIKFVETAPISIVEEICAKLKPLIF

Nucleotide

• Length: 351 bp

>T28548 NC_019748:95780-96130 [Stanieria cyanosphaera PCC 7437]
GTGAAGAAATCTGGGAGTAAACAATATATTCCGGACAGAGGCGATATTATTTGGCTAGATTTCAATCCTCAGATCGGACG
TGAGCAAGCGGGACATCGCCCTGCTATTGTTATATCTCCGAAAAAGTTTAATTCTCTTTCTTCTCTGGTTTTTGTCTGTC
CGATTAGTAGCAAAATTAAGGGATTTAGTTTTGAAGTTTTATTAACTGAACAGATGCAAACTAAAGGAGTTGTTTTAATT
CATCACCTCAGAAGCGTTGACTGGAAAACTAGAGGAATTAAATTTGTGGAAACAGCACCTATCTCAATAGTTGAAGAAAT
ATGTGCCAAGTTAAAGCCATTAATTTTCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8750.74 Da
• Isoelectric Point: 3.8960

>AT28548 WP_015191380.1 NC_019748:95560-95799 [Stanieria cyanosphaera PCC 7437]
MSTVAKWGNSLAIRIPKNIADKINLKEGTAISIDVTDTNIVITPKQPNYTLEELLAGANSEDFEGEYDWGEPVGEEIWE

Nucleotide

• Length: 240 bp

>AT28548 NC_019748:95560-95799 [Stanieria cyanosphaera PCC 7437]
ATGTCAACAGTAGCCAAATGGGGAAATAGTCTAGCGATACGCATTCCCAAAAATATTGCCGACAAAATTAATTTAAAAGA
AGGAACTGCTATTTCTATTGATGTTACTGATACCAACATTGTCATTACTCCCAAACAACCGAACTACACCCTTGAAGAAT
TACTCGCTGGAGCAAATAGTGAAGACTTTGAAGGAGAATATGATTGGGGAGAGCCAGTAGGTGAAGAAATCTGGGAGTAA

Structures

Toxin

• AlphaFold DB: K9XMI9

Antitoxin

• AlphaFold DB: K9XPV3