Detailed information of TA system TA028547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 5038477..5039104
• Replicon: chromosome
• Accession: NC_019745
• Organism: Gloeocapsa sp. PCC 7428

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GLO7428_RS22195
• Protein ID: WP_196797414.1
• Coordinates: 5038706..5039104 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: K9XLL1
• Locus tag: GLO7428_RS22190
• Protein ID: WP_015190831.1
• Coordinates: 5038477..5038704 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GLO7428_RS22165	5033878..5034696	+	819	WP_015190825.1	ABC transporter permease	-
GLO7428_RS22170	5034633..5035514	+	882	WP_196797413.1	ABC transporter permease	-
GLO7428_RS22175	5035594..5036301	+	708	WP_015190827.1	Uma2 family endonuclease	-
GLO7428_RS26975	5036711..5036986	+	276	WP_015190828.1	BrnT family toxin	-
GLO7428_RS22180	5036949..5037236	+	288	WP_015190829.1	BrnA antitoxin family protein	-
GLO7428_RS22185	5037758..5038168	+	411	WP_015190830.1	hypothetical protein	-
GLO7428_RS22190	5038477..5038704	+	228	WP_015190831.1	virulence associated protein B	Antitoxin
GLO7428_RS22195	5038706..5039104	+	399	WP_196797414.1	type II toxin-antitoxin system VapC family toxin	Toxin
GLO7428_RS22200	5039433..5039735	+	303	WP_015190833.1	hypothetical protein	-
GLO7428_RS22205	5039723..5039968	+	246	WP_015190834.1	hypothetical protein	-
GLO7428_RS22210	5040120..5041229	+	1110	WP_015190835.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
GLO7428_RS22215	5041374..5042654	+	1281	WP_015190836.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
GLO7428_RS22220	5042707..5043840	+	1134	WP_196797654.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14668.04 Da
• Isoelectric Point: 5.7109

>T28547 WP_196797414.1 NC_019745:5038706-5039104 [Gloeocapsa sp. PCC 7428]
MLLLDTNICIYLIKQKPPKVLARFRSLALSDISISSITVAELEYGVCKSQQQEKNRSALMQFLIPLEIVEFDHAAATVYG
SIRNDLESRGLVIGSMDMLIAAHALSLGVTLVSNNVREFSRISNLSIENWAE

Nucleotide

• Length: 399 bp

>T28547 NC_019745:5038706-5039104 [Gloeocapsa sp. PCC 7428]
TTGCTATTGCTTGATACCAACATTTGTATCTATCTGATTAAGCAAAAACCGCCTAAGGTACTGGCGCGATTTCGATCCCT
TGCTTTGTCAGATATCAGCATTTCTTCCATCACTGTCGCCGAATTAGAATATGGAGTTTGCAAGAGCCAACAGCAAGAGA
AAAACCGTAGTGCCTTAATGCAATTTTTAATACCTCTAGAAATTGTTGAGTTCGATCACGCTGCTGCAACCGTTTATGGC
TCGATTAGAAATGACTTGGAAAGTAGGGGGCTTGTCATTGGCTCAATGGATATGCTGATTGCTGCCCATGCCTTAAGTCT
TGGAGTTACTCTTGTAAGTAATAATGTACGCGAGTTTTCACGTATTTCTAACCTATCTATAGAAAATTGGGCGGAATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8513.63 Da
• Isoelectric Point: 4.2103

>AT28547 WP_015190831.1 NC_019745:5038477-5038704 [Gloeocapsa sp. PCC 7428]
MNTAKISTDGTHQIVILPEEFQITGTEVYIKKVGNAIVLIAKDNPWQSLFESLEQFSEDFMTTREQPSLDVREAL

Nucleotide

• Length: 228 bp

>AT28547 NC_019745:5038477-5038704 [Gloeocapsa sp. PCC 7428]
ATGAATACAGCCAAAATTAGCACCGATGGTACTCATCAAATTGTCATATTACCTGAAGAATTTCAAATAACTGGCACTGA
GGTTTACATTAAGAAAGTTGGTAATGCGATCGTTCTCATCGCTAAAGATAATCCTTGGCAATCTTTGTTTGAAAGCTTAG
AGCAATTCTCTGAAGATTTCATGACTACTAGAGAACAGCCCTCCCTCGATGTGCGAGAGGCTTTGTAG

Structures

Antitoxin

• AlphaFold DB: K9XLL1