Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2982941..2983595 Replicon chromosome
Accession NZ_LM995446
Organism Escherichia coli strain K-12 substr. RV308

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag ECRV308_RS14780 Protein ID WP_000244777.1
Coordinates 2982941..2983348 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECRV308_RS14785 Protein ID WP_000354046.1
Coordinates 2983329..2983595 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECRV308_RS14760 2978898..2980631 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
ECRV308_RS14765 2980637..2981347 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECRV308_RS14770 2981372..2982268 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECRV308_RS14775 2982380..2982901 + 522 WP_001055874.1 flavodoxin FldB -
ECRV308_RS14780 2982941..2983348 - 408 WP_000244777.1 toxin CptA Toxin
ECRV308_RS14785 2983329..2983595 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECRV308_RS14790 2983838..2984818 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
ECRV308_RS14795 2985014..2985673 - 660 WP_000250274.1 hemolysin III family protein -
ECRV308_RS14800 2985837..2986148 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
ECRV308_RS14805 2986193..2987626 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
ECRV308_RS14810 2987683..2988426 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T285445 WP_000244777.1 NZ_LM995446:c2983348-2982941 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT285445 WP_000354046.1 NZ_LM995446:c2983595-2983329 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References